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Biocompatibility ev...
Biocompatibility evaluation of densified bacterial nanocellulose hydrogel as an implant material for auricular cartilage regeneration
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- Martinez Avila, Hector, 1985 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Schwarz, S. (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Feldmann, Eva-Maria (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Mantas, Athanasios, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- von Bomhard, A. (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Gatenholm, Paul, 1956 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Rotter, N. (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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(creator_code:org_t)
- 2014-05-28
- 2014
- Engelska.
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Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 1432-0614 .- 0175-7598. ; 98:17, s. 7423-7435
- Relaterad länk:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Bacterial nanocellulose (BNC), synthesized by the bacterium Gluconacetobacter xylinus, is composed of highly hydrated fibrils (99 % water) with high mechanical strength. These exceptional material properties make BNC a novel biomaterial for many potential medical and tissue engineering applications. Recently, BNC with cellulose content of 15 % has been proposed as an implant material for auricular cartilage replacement, since it matches the mechanical requirements of human auricular cartilage. This study investigates the biocompatibility of BNC with increased cellulose content (17 %) to evaluate its response in vitro and in vivo. Cylindrical BNC structures (48 Au 20 mm) were produced, purified in a built-in house perfusion system, and compressed to increase the cellulose content in BNC hydrogels. The reduction of endotoxicity of the material was quantified by bacterial endotoxin analysis throughout the purification process. Afterward, the biocompatibility of the purified BNC hydrogels with cellulose content of 17 % was assessed in vitro and in vivo, according to standards set forth in ISO 10993. The endotoxin content in non-purified BNC (2,390 endotoxin units (EU)/ml) was reduced to 0.10 EU/ml after the purification process, level well below the endotoxin threshold set for medical devices. Furthermore, the biocompatibility tests demonstrated that densified BNC hydrogels are non-cytotoxic and cause a minimal foreign body response. In support with our previous findings, this study concludes that BNC with increased cellulose content of 17 % is a promising non-resorbable biomaterial for auricular cartilage tissue engineering, due to its similarity with auricular cartilage in terms of mechanical strength and host tissue response.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
Nyckelord
- CHONDROCYTES
- SYSTEMS
- Endotoxin analysis
- Bacterial cellulose
- CELLULOSE SCAFFOLDS
- POTENTIAL SCAFFOLD
- BEHAVIOR
- ACETOBACTER-XYLINUM
- Auricular cartilage tissue
- INFLAMMATION
- Depyrogenation
- PLA
- Biocompatibility
- RESPONSES
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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