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The L2 Loop Peptide...
The L2 Loop Peptide of recA Stiffens and restricts Base Motions of Single-stranded DNA Similar to Intact Protein
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Selmane, T. (author)
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- Wittung Stafshede, Pernilla, 1968 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Maraboeuf, F. (author)
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Voloshin, O. (author)
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- Nordén, Bengt, 1945 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Camerini-Otero, D. (author)
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Takahashi, M. (author)
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(creator_code:org_t)
- 1999
- 1999
- English.
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In: FEBS Letters. - 1873-3468 .- 0014-5793. ; 446:1, s. 30-34
- Related links:
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http://dx.doi.org/10...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The L2 loop in the RecA protein is the catalytic center for DNA strand exchange, Here we investigate the DMA binding properties of the L2 loop peptide using optical spectroscopy with polarized light. Both fluorescence intensity and anisotropy of an etheno-modified poly(dA) increase upon peptide binding, indicate that the base motions of single-stranded DNA are restricted in the complex. In agreement with this conclusion, the peptide-poly(dT) complex exhibits a significant linear dichroism signal. The peptide is also found to modify the structure of double-stranded DNA, but does not denature it. It is inferred that strand separation may not be required for the formation of a joint molecule.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Keyword
- DNA binding
- peptide
- homologous recombination
- RecA protein
- DNA base motion
Publication and Content Type
- art (subject category)
- ref (subject category)
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