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Pichia protocols /
Pichia protocols / edited by James M. Cregg.
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Cregg, James M. (författare)
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Cregg, James M. (redaktör/utgivare)
- ISBN 9781588294296
- 2. ed.
- Totowa, N.J. Humana Press, cop. 2007
- Engelska xi, 268 p.
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Serie: Methods in molecular biology ; 389
- Relaterad länk:
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http://www.loc.gov/c... (Table of contents)
Innehållsförteckning
Abstract
Recensioner
Ämnesord
Stäng
- Introduction : distinction between Pichia pastoris and other expression systems / James M. Cregg -- Vectors and strains for expression / Joan Lin-Cereghino and Geoff P. Lin-Cereghino -- DNA-mediated transformation / James M. Cregg -- Rational design and optimization of fed-batch and continuous fermentation / Wenhui Zhag, Mehmet Inan, and Michael M. Meagher -- Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1) / Mehmet Inan ... [et al.] -- Purification of the N- and C-terminal subdomains of recombinant heavy chain fragment C of botulinum neurotoxin serotype C / Jicai Huang ... [et al.] -- Rapid screening of chromatography resins for the purification of proteins / Sandra E. Ríos, Erin M. Giaccone, and Tillman U. Gerngross -- Characterization of O-linked saccharides on glycoproteins / Roger K. Bretthauer -- Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using glycoswitch plasmids / Wouter Vervecken ... [et al.] -- N-linked glycan characterization of heterologous proteins / Huijan Li ... [et al.] -- Heavy labeling of recombinant proteins / Eric Rodriguez -- Selenomethionine labeling of recombinant proteins / Anna M. Larsson and T. Alwyn Jones -- Selective isotopic labeling of recombinant proteins using amino acid auxotroph strains / James W. Whittaker -- Classical genetics / Ilya Tolstorukov and James M. Cregg -- Identification of pexophagy genes by restriction enzyme-mediated integration / Laura A. Schroder, Benjamin S. Glick, and William A. Dunn, Jr. -- Characterization of protein-protein interactions : application to the understanding of peroxisome biogenesis / Sebastien Leon ... [et al.] -- Localization of proteins and organelles using fluorescence microscopy / Jean-Claude Farre ... [et al.] -- Fluorescence microscopy and thin-section electron microscopy / Benjamin S. Glick.
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Ämnesord
- Pichia pastoris. (LCSH)
- Genetic vectors. (LCSH)
- Yeast fungi -- Biotechnology. (LCSH)
- Pichia -- genetics -- Laboratory Manuals. (MeSH)
- Recombinant Proteins -- Laboratory Manuals. (MeSH)
Nyckelord
- Uec.045 Mikrobiologi: genetik
- Ufhe Svampar (Fungi)
Publikations- och innehållstyp
- TP248.27.Y43 (LCC)
- W1 (NLM)
- QW 25 (NLM)
- 660.6/3 (DDC)
- 660.62 (DDC)
- Uec.045 (kssb/8)
- Ufhe (kssb/8)
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