A suggested new bacteriophage genus, “Kp34likevirus”, within the Autographivirinae subfamily of Podoviridae

• Klebsiella pneumoniae  phages vB_KpnP_SU503 (SU503) and vB_KpnP_SU552A (SU552A) are virulent viruses belonging to the Autographivirinae  subfamily of  Podoviridae  that infect and kill multi-resistant  K. pneumoniae  isolates. Phages SU503 and SU552A show high pairwise nucleotide identity to  Klebsiella  phages KP34 (NC_013649), F19 (NC_023567) and NTUH-K2044-K1-1 (NC_025418). Bioinformatic analysis of these phage genomes show high conservation of gene arrangement and gene content, conserved catalytically active residues of their RNA polymerase, a common and specific lysis cassette, and form a joint cluster in phylogenetic analysis of their conserved genes. Also, we have performed biological characterization of the burst size, latent period, host specificity (together with KP34 and NTUH-K2044-K1-1), morphology, and structural genes as well as sensitivity testing to various conditions. Based on the analyses of these phages, the creation of a new phage genus is suggested within the  Autographivirinae , called “Kp34likevirus” after their type phage, KP34. This genus should encompass the recently genome sequenced  Klebsiella  phages KP34, SU503, SU552A, F19 and NTUH-K2044-K1-1.

Ämnesord

Natural Sciences  (hsv)

Biological Sciences  (hsv)

Genetics  (hsv)

Naturvetenskap  (hsv)

Biologiska vetenskaper  (hsv)

Genetik  (hsv)

molekylärgenetik  (su)

Molecular Genetics  (su)

Nyckelord

“Kp34likevirus”

phikmvlikevirus

Autographivirinae

Klebsiella pneumoniae

bacteriophage

phage

Podoviridae