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Imaging sub-plasma ...
Imaging sub-plasma membrane cAMP dynamics with fluorescent translocation reporters
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Tengholm, Anders 1971- (författare)
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Idevall-Hagren, Olof (författare)
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(utgivare)
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(medarbetare)
- Springer-Verlag New York 2015
- 2015
- Engelska.
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Serie: Methods in Molecular Biology 1064-3745
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Ingår i: cAMP Signaling. ; 85-101
Abstract
Ämnesord
Stäng
- Imaging cAMP dynamics in single cells and tissues can provide important insights into the regulation of a variety of cellular processes. In recent years, a large number of tools for cAMP measurements have been developed. While most cAMP reporters are designed to undergo changes in fluorescence resonance energy transfer (FRET), there are alternative techniques with advantages for certain applications. Here, we describe protocols for cAMP measurements in the sub-plasma membrane space based on the detection of the cAMP-induced translocation of engineered fluorescent protein-tagged subunits of protein kinase A between the cytoplasm and the plasma membrane. Total internal reflection fluorescence (TIRF) imaging of the changes in reporter localization yields robust signal changes and has contributed to the discovery of cAMP oscillations in the sub-plasma membrane space of insulin-secreting β-cells stimulated with glucose and gluco-incretin hormones. We also demonstrate how the technique can be combined with measurements of the cytosolic Ca 2+ concentration or with recordings of the subcellular localization of the cAMP effector protein Epac2. The translocation reporter approach provides a valuable complement to other methods for imaging sub-membrane cAMP dynamics in various types of cells.
Ämnesord
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Cell and Molecular Biology (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska grundvetenskaper (hsv)
- Cell- och molekylärbiologi (hsv)
- Medical Cell Biology (uu)
- Medicinsk cellbiologi (uu)
Nyckelord
- cAMP oscillations; Plasma membrane; Protein kinase A; Translocation; Total internal reflection fluorescence