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Cellular responses ...
Cellular responses to T-2 toxin and/or deoxynivalenol that induce cartilage damage are not specific to chondrocytes
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Lei, Yang (författare)
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Guanghui, Zhao (författare)
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Xi, Wang (författare)
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visa fler...
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Yingting, Wang (författare)
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Xialu, Lin (författare)
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Fangfang, Yu (författare)
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Goldring, Mary (författare)
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Xiong, Guo (författare)
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Lammi, Mikko 1961- (författare)
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visa färre...
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(utgivare)
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(medarbetare)
- London Nature Publishing Group 2017
- 2017
- Engelska.
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Ingår i: Scientific Reports. - 2045-2322. ; 7:1, 2231
Abstract
Ämnesord
Stäng
- The relationship between T-2 toxin and deoxynivalenol (DON) and the risk of Kashin-Beck disease is still controversial since it is poorly known about their selectivity in cartilage damage. We aimed to compare the cytotoxicity of T-2 toxin and DON on cell lines representative of cell types encountered in vivo, including human chondrocytes (C28/I2), human hepatic epithelial cells (L-02) and human tubular epithelial cells (HK-2). In addition, we determined the distribution of T-2 toxin and DON in Sprague-Dawley (SD) rats after a single dose exposure. T-2 toxin or DON decreased proliferation in a time- and concentration-dependent manner and their combination showed a similar antagonistic effect in C28/I2, L-02 and HK-2 cells. Moreover, we observed cell cycle arrest and apoptosis, associated with increased oxidative stress and decline in mitochondrial membrane potential induced by T-2 toxin and/or DON. In vivo study showed that T-2 toxin and DON did not accumulate preferentially in the knee joint compared to liver and kidney after an acute exposure in SD rats. These results suggest that T-2 toxin and/or DON inhibit proliferation and induce apoptosis through a possible mechanism involving reactive oxygen species-mediated mitochondrial pathway that is not specific for chondrocytes in vitro or joint tissues in vivo.
Ämnesord
- Medical and Health Sciences (hsv)
- Clinical Medicine (hsv)
- Orthopedics (hsv)
- Medicin och hälsovetenskap (hsv)
- Klinisk medicin (hsv)
- Ortopedi (hsv)
- Basic Medicine (hsv)
- Pharmacology and Toxicology (hsv)
- Medicinska grundvetenskaper (hsv)
- Farmakologi och toxikologi (hsv)
- Cell and Molecular Biology (hsv)
- Cell- och molekylärbiologi (hsv)
- cellforskning (umu)
- cellforskning (umu)
- Medical Cell Biology (umu)
- medicinsk cellbiologi (umu)
- Pathology (umu)
- patologi (umu)
- Toxicology (umu)
- toxikologi (umu)
Nyckelord
- Kashin-Beck disease
- T-2 toxin
- deoxynivalenol
- chondrocyte
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