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  • Nilsson, Mikael T. (författare)

Structural basis for the inhibition of Mycobacterium tuberculosis glutamine synthetase by novel ATP-competitive inhibitors

  • E-artikel/E-kapitelEngelska2009

Förlag, utgivningsår, omfång ...

  • 2009

Nummerbeteckningar

  • LIBRIS-ID:22159470
  • http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-97546uri
  • urn:nbn:se:uu:diva-97546urn
  • 10.1016/j.jmb.2009.08.028doi

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  • Språk:engelska

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Anmärkningar

  • Published
  • gratis
  • Glutamine synthetase (GS, EC 6.3.1.2; also known as γ-glutamyl:ammonia ligase) catalyzes the ATP-dependent condensation of glutamate and ammonia to form glutamine. The enzyme has essential roles in different tissues and species, which have led to its consideration as a drug or an herbicide target. In this article, we describe studies aimed at the discovery of new antimicrobial agents targeting Mycobacterium tuberculosis, the causative pathogen of tuberculosis. A number of distinct classes of GS inhibitors with an IC 50 of micromolar value or better were identified via high-throughput screening. A commercially available purine analogue similar to one of the clusters identified (the diketopurines), 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-dione, was also shown to inhibit the enzyme, with a measured IC 50 of 2.5 ± 0.4 μM. Two X-ray structures are presented: one is a complex of the enzyme with the purine analogue alone (2.55-Å resolution), and the other includes the compound together with methionine sulfoximine phosphate, magnesium and phosphate (2.2-Å resolution). The former represents a relaxed, inactive conformation of the enzyme, while the latter is a taut, active one. These structures show that the compound binds at the same position in the nucleotide site, regardless of the conformational state. The ATP-binding site of the human enzyme differs substantially, explaining why it has an ∼ 60-fold lower affinity for this compound than the bacterial GS. As part of this work, we devised a new synthetic procedure for generating l-(SR)-methionine sulfoximine phosphate from l-(SR)-methionine sulfoximine, which will facilitate future investigations of novel GS inhibitors.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Krajewski, Wojciech W. (författare)
  • Srinivasa, Bachally R. (författare)
  • Yahiaoui, Samir (författare)
  • Larhed, Mats (författare)
  • Karlén, Anders (författare)
  • Jones, T. Alwyn (författare)
  • Mowbray, Sherry L. (författare)
  • Uppsala universitetTeknisk-naturvetenskapliga vetenskapsområdet (utgivare)
  • Uppsala universitetMedicinska och farmaceutiska vetenskapsområdet (utgivare)
  • Uppsala universitetMedicinska och farmaceutiska vetenskapsområdet (utgivare)

Sammanhörande titlar

  • Del av/supplement till:channel record
  • Ingår i:VärdpublikationJournal of Molecular Biology393:2, 504-5130022-2836

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