Sökning: WFRF:(Mathai Benan J.) > HS1BP3 negatively r...
Fältnamn | Indikatorer | Metadata |
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000 | 03356naa a22006253a 4500 | |
001 | 20000645 | |
003 | SE-LIBR | |
005 | 20170118155022.0 | |
007 | cr|||||||||||| | |
008 | 170118s2016 sw |||| o |||| ||eng c | |
024 | 7 | a http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1302212 uri |
024 | 7 | a urn:nbn:se:umu:diva-1302212 urn |
024 | 7 | a 10.1038/ncomms138892 doi |
040 | a S | |
041 | 0 | a eng |
042 | 9 EPLK | |
100 | 1 | a Holland, Petter4 aut |
245 | 1 0 | a HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levelsh [Elektronisk resurs] |
260 | c 2016 | |
500 | a Published | |
506 | 0 | a gratis |
520 | a A fundamental question is how autophagosome formation is regulated. Here we show that the PX domain protein HS1BP3 is a negative regulator of autophagosome formation. HS1BP3 depletion increased the formation of LC3-positive autophagosomes and degradation of cargo both in human cell culture and in zebrafish. HS1BP3 is localized to ATG16L1-and ATG9-positive autophagosome precursors and we show that HS1BP3 binds phosphatidic acid (PA) through its PX domain. Furthermore, we find the total PA content of cells to be significantly upregulated in the absence of HS1BP3, as a result of increased activity of the PA-producing enzyme phospholipase D (PLD) and increased localization of PLD1 to ATG16L1-positive membranes. We propose that HS1BP3 regulates autophagy by modulating the PA content of the ATG16L1-positive autophagosome precursor membranes through PLD1 activity and localization. Our findings provide key insights into how autophagosome formation is regulated by a novel negative-feedback mechanism on membrane lipids. | |
650 | 7 | a Medical and Health Sciences2 hsv |
650 | 7 | a Basic Medicine2 hsv |
650 | 7 | a Cell and Molecular Biology2 hsv |
650 | 7 | a Medicin och hälsovetenskap2 hsv |
650 | 7 | a Medicinska grundvetenskaper2 hsv |
650 | 7 | a Cell- och molekylärbiologi2 hsv |
700 | 1 | a Knaevelsrud, Helene4 aut |
700 | 1 | a Soreng, Kristiane4 aut |
700 | 1 | a Mathai, Benan J.4 aut |
700 | 1 | a Lystad, Alf Hakon4 aut |
700 | 1 | a Pankiv, Serhiy4 aut |
700 | 1 | a Bjorndal, Gunnveig T.4 aut |
700 | 1 | a Schultz, Sebastian W.4 aut |
700 | 1 | a Lobert, Viola H.4 aut |
700 | 1 | a Chan, Robin B.4 aut |
700 | 1 | a Zhou, Bowen4 aut |
700 | 1 | a Liestol, Knut4 aut |
700 | 1 | a Carlsson, Sven R.4 aut |
700 | 1 | a Melia, Thomas J.4 aut |
700 | 1 | a Di Paolo, Gilbert4 aut |
700 | 1 | a Simonsen, Anne4 aut |
710 | 1 2 | a Umeå universitetb Medicinska fakulteten4 pbl0 268483 |
772 | 1 8 | i channel recordw 18813935 |
773 | 0 8 | i Värdpublikationt Nature Communicationsg 7x 2041-1723 |
856 | 4 0 | u http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130221 |
856 | 4 0 | u http://dx.doi.org/10.1038/ncomms13889 |
856 | 4 0 | u http://umu.diva-portal.org/smash/get/diva2:1066077/FULLTEXT01 |
910 | 2 s | 6 710a Umeå universitet.b Medicinsk-odontologiska fakultetenu Umeå universitet.b Medicinska fakulteten |
910 | 2 s | 6 710a Medicinska fakulteten vid Umeå universitetu Umeå universitet.b Medicinska fakulteten |
841 | 5 APISa x ab 170118||0000|||||001||||||000000e 1 | |
024 | 7 | 5 APISa urn:nbn:se:umu:diva-1302212 urn |
852 | 5 APISb APIS | |
856 | 4 0 | 5 APISu http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130221 |
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