WFRF:(Di Paolo Gilbert)
Sökning: WFRF:(Di Paolo Gilbert) > A novel role for ph...
- Elvers, Margitta (författare)
A novel role for phospholipase D as an endogenous negative regulator of platelet sensitivity
- E-artikel/E-kapitelEngelska2012
Förlag, utgivningsår, omfång ...
- Elsevier2012
Nummerbeteckningar
- LIBRIS-ID:22108603
- http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-80685uri
- urn:nbn:se:liu:diva-80685urn
- 10.1016/j.cellsig.2012.04.018doi
Kompletterande språkuppgifter
- Språk:engelska
Ingår i deldatabas
Anmärkningar
- Published
- gratis
- Platelet aggregation, secretion and thrombus formation play a critical role in primary hemostasis to prevent excessive blood loss. On the other hand, uncontrolled platelet activation leads to pathological thrombus formation resulting in myocardial infarction or stroke. Stimulation of heterotrimeric G-proteins by soluble agonists or immunoreceptor tyrosine based activation motif-coupled receptors that interact with immobilized ligands such as the collagen receptor glycoprotein (GP) VI lead to the activation of phospholipases that cleave membrane phospholipids to generate soluble second messengers. Platelets contain the phospholipases (PL) D1 and D2 which catalyze the hydrolysis of phosphatidylcholine to generate the second messenger phosphatidic acid (PA). The production of PA is abrogated by primary alcohols that have been widely used for the analysis of PLD-mediated processes. However, it is not clear if primary alcohols effectively reduce PA generation or if they induce PLD-independent cellular effects. In the present study we made use of the specific PLD inhibitor 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) and show for the first time, that FIPI enhances platelet dense granule secretion and aggregation of human platelets. Further. FIPI has no effect on cytosolic Ca2+ activity but needs proper Rho kinase signaling to mediate FIPI-induced effects on platelet activation. Upon FIPI treatment the phosphorylation of the PKC substrate pleckstrin was prominently enhanced suggesting that FIPI affects PKC-mediated secretion and aggregation in platelets. Similar effects of FIPI were observed in platelets from mouse wild-type and Pld1(-/-) mice pointing to a new role for PLD2 as a negative regulator of platelet sensitivity.
Ämnesord och genrebeteckningar
- Medical and Health Sciences hsv
- Medicin och hälsovetenskap hsv
- MEDICINE svep
- MEDICIN svep
- PLD; Platelets; Secretion; Aggregation; Regulation
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Grenegård, Magnus (författare)
- Khoshjabinzadeh, Hanieh (författare)
- Muenzer, Patrick (författare)
- Borst, Oliver (författare)
- Tian, Huasong (författare)
- Di Paolo, Gilbert (författare)
- Lang, Florian (författare)
- Gawaz, Meinrad (författare)
- Lindahl, Tomas (författare)
- Fälker, Knut (författare)
- Linköpings universitetInstitutionen för medicin och hälsa308697 (utgivare)
- Linköpings universitetHälsouniversitetet (utgivare)
- Linköpings universitetInstitutionen för klinisk och experimentell medicin347333 (utgivare)
- Östergötlands Läns LandstingDiagnostikcentrum (utgivare)
- Linköpings universitetInstitutionen för klinisk och experimentell medicin347333 (utgivare)
Sammanhörande titlar
- Del av/supplement till:channel record
- Ingår i:VärdpublikationCellular Signalling24:9, 1743-17520898-6568
Internetlänk
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
