PDGF-A and PDGF-B induces cardiac fibrosis in transgenic mice

doi:10.1016/j.yexcr.2016.10.022

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Betsholtz Christer) > PDGF-A and PDGF-B i...

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		03893naa a22006613a 4500
001		20188375
003		SE-LIBR
005		20170322175937.0
007		cr\|\|\|\|\|\|\|\|\|\|\|\|
008		170322s2016 sw \|\|\|\| o \|\|\|\| \|\|eng c
024	7	a http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3120372 uri
024	7	a urn:nbn:se:uu:diva-3120372 urn
024	7	a 10.1016/j.yexcr.2016.10.0222 doi
040		a S
041	0	a eng
042		9 EPLK
100	1	a Gallini, Radiosa4 aut
245	1 0	a PDGF-A and PDGF-B induces cardiac fibrosis in transgenic miceh [Elektronisk resurs]
260		c 2016
500		a Published
500		a Cancerfonden
500		a Vetenskapsrådet
500		a Novo Nordisk
500		a Knut och Alice Wallenbergs Stiftelse
500		a Torsten Söderbergs stiftelse
500		a Ragnar Söderbergs stiftelse
506	0	a gratis
520		a Platelet-derived growth factors (PDGFs) and their receptors (PDGFRs) contribute to normal heart development. Deficient or abnormal expression of Pdgf and Pdgfr genes have a negative impact on cardiac development and function. The cellular effects of PDGFs in the hearts of Pdgf/Pdgfr mutants and the pathogenesis of the resulting abnormalities are poorly understood, but different PDGF isoforms induce varying effects. Here, we generated three new transgenic mouse types which complete a set of studies, where all different PDGF ligands have been expressed under the same heart specific alpha-myosin heavy chain promoter. Transgenic expression of the natural isoforms of Pdgfa and Pdgfb resulted in isoform specific fibrotic reactions and cardiac hypertrophy. Pdgfa overexpression resulted in a severe fibrotic reaction with up to 8-fold increase in cardiac size, leading to lethal cardiac failure within a few weeks after birth. In contrast, Pdgfb overexpression led to focal fibrosis and moderate cardiac hypertrophy. As PDGF-A and PDGF-B have different affinity for the two PDGF receptors, we analyzed the expression of the receptors and the histology of the fibrotic hearts. Our data suggest that the stronger fibrotic effect generated by Pdgfa overexpression was mediated by Pdgfra in cardiac interstitial mesenchymal cells, i.e. the likely source of extracellular matrix depostion and fibrotic reaction. The apparent sensitivity of the heart to ectopic PDGFR alpha agonists supports a role for endogenous PDGFRa agonists in the pathogenesis of cardiac fibrosis.
650	7	a Medical and Health Sciences2 hsv
650	7	a Clinical Medicine2 hsv
650	7	a Cancer and Oncology2 hsv
650	7	a Medicin och hälsovetenskap2 hsv
650	7	a Klinisk medicin2 hsv
650	7	a Cancer och onkologi2 hsv
650	7	a Basic Medicine2 hsv
650	7	a Cell and Molecular Biology2 hsv
650	7	a Medicinska grundvetenskaper2 hsv
650	7	a Cell- och molekylärbiologi2 hsv
653	0	a PDGF
653	0	a Transgene
653	0	a Myosin heavy chain
653	0	a Fibrosis
653	0	a Heart
700	1	a Lindblom, Per4 aut
700	1	a Bondjers, Cecilia4 aut
700	1	a Betsholtz, Christer4 aut
700	1	a Andrae, Johanna4 aut
710	1 2	a Uppsala universitetb Medicinska och farmaceutiska vetenskapsområdet4 pbl
710	1 2	a Uppsala universitetb Medicinska och farmaceutiska vetenskapsområdet4 pbl
772	1 8	i channel recordw 19753896
773	0 8	i Värdpublikationt Experimental Cell Researchg 349:2, 282-290x 0014-4827
856	4 0	u http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-312037
856	4 0	u http://dx.doi.org/10.1016/j.yexcr.2016.10.022
856	4 0	u http://uu.diva-portal.org/smash/get/diva2:1062375/FULLTEXT01
841		5 APISa x ab 170322\|\|0000\|\|\|\|\|001\|\|\|\|\|\|000000e 1
024	7	5 APISa urn:nbn:se:uu:diva-3120372 urn
852		5 APISb APIS
856	4 0	5 APISu http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-312037

Hitta via bibliotek

Experimental Cell Research (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Gallini, Radiosa; Lindblom, Per; Bondjers, Cecili ...; Betsholtz, Chris ...; Andrae, Johanna

Av lärosätet: Swepub_uni:_t

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se