A genome wide dosage suppressor network reveals genomic robustness

• Genomic robustness is the extent to which an organism has evolved to withstand the effects of deleterious mutations. We explored the extent of genomic robustness in budding yeast by genome wide dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesis related genes, revealing 660 suppressor interactions of which 642 are novel. This collection has several distinctive features, including high cooccurrence of mutant-suppressor pairs within protein modules, highly correlated functions between the pairs and higher diversity of functions among the co-suppressors than previously observed. Dosage suppression of essential genes encoding RNA polymerase subunits and chromosome cohesion complex suggests a surprising degree of functional plasticity of macromolecular complexes, and the existence of numerous degenerate pathways for circumventing the effects of potentially lethal mutations. These results imply that organisms and cancer are likely able to exploit the genomic robustness properties, due the persistence of cryptic gene and pathway functions, to generate variation and adapt to selective pressures. © 2016 The Author(s).

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

RNA polymerase

RNA polymerase II

Saccharomyces cerevisiae protein

Article

bacterial strain

cell cycle regulation

cell division

correlational study

DNA replication

gene expression

gene function

gene interaction

gene repression

genetic analysis

genetic parameters

genome wide dosage suppressor

genomic robustness

microarray analysis

mutant

protein analysis

protein interaction

RNA processing

RNA synthesis

Saccharomyces cerevisiae

biology

fungal genome

gene dosage

gene expression profiling

gene expression regulation

gene regulatory network

genetics

lethal gene

metabolism

mutation

reproductive fitness

Computational Biology

Gene Expression Regulation

Fungal

Gene Regulatory Networks

Genes

Lethal

Genetic Fitness

Genome

Fungal

Saccharomyces cerevisiae Proteins

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)