A genome wide dosage suppressor network reveals genomic robustness

Genomic robustness is the extent to which an organism has evolved to withstand the effects of deleterious mutations. We explored the extent of genomic robustness in budding yeast by genome wide dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesis related genes, revealing 660 suppressor interactions of which 642 are novel. This collection has several distinctive features, including high cooccurrence of mutant-suppressor pairs within protein modules, highly correlated functions between the pairs and higher diversity of functions among the co-suppressors than previously observed. Dosage suppression of essential genes encoding RNA polymerase subunits and chromosome cohesion complex suggests a surprising degree of functional plasticity of macromolecular complexes, and the existence of numerous degenerate pathways for circumventing the effects of potentially lethal mutations. These results imply that organisms and cancer are likely able to exploit the genomic robustness properties, due the persistence of cryptic gene and pathway functions, to generate variation and adapt to selective pressures. © 2016 The Author(s).

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
RNA polymerase
RNA polymerase II
Saccharomyces cerevisiae protein
Article
bacterial strain
cell cycle regulation
cell division
correlational study
DNA replication
gene expression
gene function
gene interaction
gene repression
genetic analysis
genetic parameters
genome wide dosage suppressor
genomic robustness
microarray analysis
mutant
protein analysis
protein interaction
RNA processing
RNA synthesis
Saccharomyces cerevisiae
biology
fungal genome
gene dosage
gene expression profiling
gene expression regulation
gene regulatory network
genetics
lethal gene
metabolism
mutation
reproductive fitness
Computational Biology
Gene Expression Regulation
Fungal
Gene Regulatory Networks
Genes
Lethal
Genetic Fitness
Genome
Fungal
Saccharomyces cerevisiae Proteins

Kon, Y. (författare)
Yadav, G. (författare)
Sevold, A. W. (författare)
Frumkin, J. P. (författare)
Vallabhajosyula, R. R. (författare)
Hintze, Arend,ProfessorKeck Graduate Institute of Applied Life Sciences, Claremont, United States(Swepub:du)ahz (författare)
Stman, B. (författare)
Schossau, J. (författare)
Bhan, A. (författare)
Marzolf, B. (författare)
Tamashiro, J. K. (författare)
Kaur, A. (författare)
Baliga, N. S. (författare)
Grayhack, E. J. (författare)
Adami, C. (författare)
Galas, D. J. (författare)
Raval, A. (författare)
Phizicky, E. M. (författare)
Ray, A. (författare)
Keck Graduate Institute of Applied Life Sciences, Claremont, United States (creator_code:org_t)

Ingår i:Nucleic Acids Research: Oxford University Press45:1, s. 255-2700305-10481362-4962

Av författaren/redakt...: Patra, B.; Kon, Y.; Yadav, G.; Sevold, A. W.; Frumkin, J. P.; Vallabhajosyula, ...; visa fler...; Hintze, Arend, P ...; Stman, B.; Schossau, J.; Bhan, A.; Marzolf, B.; Tamashiro, J. K.; Kaur, A.; Baliga, N. S.; Grayhack, E. J.; Adami, C.; Galas, D. J.; Raval, A.; Phizicky, E. M.; Ray, A.; visa färre...

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