A Possible Industrial Solution to Ferment Lignocellulosic Hydrolyzate to Ethanol : Continuous Cultivation with Flocculating Yeast

• Cultivation of toxic lignocellulosic hydrolyzates has been a research topic in recent decades. Although several methods have been proposed, there has been doubt about their industrial applications. The current work deals with a solution to this problem which has a good potential application in industrial scale. A toxic dilute-acid hydrolyzate was continuously cultivated using a high-cell-density flocculating yeast in a single and serial bioreactor which was equipped with a settler to recycle the cells back to the bioreactors. No prior detoxification was necessary to cultivate the hydrolyzates, as the flocks were able to detoxify it in situ. The experiments were successfully carried out at dilution rates up to 0.52 h-1. The cell concentration inside the bioreactors was between 23 and 35 g-DW/L, while this concentration in the effluent of the settlers was 0.320.05 g-DW/L. The ethanol yield of 0.42-0.46 g/g-consumed sugar was achieved, and the residual sugar concentration was less than 6% of the initial fermentable sugar (glucose, galactose and mannose) of 35.2 g/L.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)

Nyckelord

dilute-acid lignocellulosic hydrolysate

scharomyces cerevisiae

flocculting yeast

ethanol

HMF

furfural

continuous cultivation

Energi och material

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)