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  • Kolossov, EugenAxiogenesis AG, 50931 Cologne, Germany (author)

Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium

  • Article/chapterEnglish2006

Publisher, publication year, extent ...

  • 2006-09-05
  • New York, USA :Rockefeller University Press,2006
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:hh-15174
  • https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-15174URI
  • https://doi.org/10.1084/jem.20061469DOI
  • https://lup.lub.lu.se/record/389540URI

Supplementary language notes

  • Language:English
  • Summary in:English

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Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Cellular cardiomyoplasty is an attractive option for the treatment of severe heart failure. It is, however, still unclear and controversial which is the most promising cell source. Therefore, we investigated and examined the fate and functional impact of bone marrow (BM) cells and embryonic stem cell (ES cell)-derived cardiomyocytes after transplantation into the infarcted mouse heart. This proved particularly challenging for the ES cells, as their enrichment into cardiomyocytes and their long-term engraftment and tumorigenicity are still poorly understood. We generated transgenic ES cells expressing puromycin resistance and enhanced green fluorescent protein cassettes under control of a cardiac-specific promoter. Puromycin selection resulted in a highly purified (>99%) cardiomyocyte population, and the yield of cardiomyocytes increased 6-10-fold because of induction of proliferation on purification. Long-term engraftment (4-5 months) was observed when co-transplanting selected ES cell-derived cardiomyocytes and fibroblasts into the injured heart of syngeneic mice, and no teratoma formation was found (n = 60). Although transplantation of ES cell-derived cardiomyocytes improved heart function, BM cells had no positive effects. Furthermore, no contribution of BM cells to cardiac, endothelial, or smooth muscle neogenesis was detected. Hence, our results demonstrate that ES-based cell therapy is a promising approach for the treatment of impaired myocardial function and provides better results than BM-derived cells.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Bostani, ToktamInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Roell, WilhelmDepartment of Cardiac Surgery, University of Bonn, 53105 Bonn, Germany (author)
  • Breitbach, MartinInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Pillekamp, FrankInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Nygren, Jens Martin,1976-Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden(Swepub:lu)stem-jny (author)
  • Sasse, PhilippInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Rubenchik, OlgaInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Fries, Jochen W UDepartment Pathology, University of Cologne, 50931 Cologne, Germany (author)
  • Wenzel, DanielaInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Geisen, CarolineInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Xia, YingInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Lu, ZhongjuInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Duan, YaqiInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Kettenhofen, RalfAxiogenesis AG, 50931 Cologne, Germany (author)
  • Jovinge, StefanLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden(Swepub:lu)medf-sjo (author)
  • Bloch, WilhelmGerman Sports University, 50927 Cologne, Germany (author)
  • Bohlen, HeribertAxiogenesis AG, 50931 Cologne, Germany (author)
  • Welz, ArminDepartment of Cardiac Surgery, University of Bonn, 53105 Bonn, Germany (author)
  • Hescheler, JürgenInstitute of Neurophysiology, University of Cologne, 50931 Cologne, Germany (author)
  • Jacobsen, Sten Eirik WLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden(Swepub:lu)stem-sja (author)
  • Fleischmann, Bernd KInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (author)
  • Axiogenesis AG, 50931 Cologne, GermanyInstitute of Physiology I, University of Bonn, 53105 Bonn, Germany (creator_code:org_t)

Related titles

  • In:Journal of Experimental MedicineNew York, USA : Rockefeller University Press203:10, s. 2315-23270022-10071540-9538

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