L773:1873 376X OR L773:1570 0232
Search: L773:1873 376X OR L773:1570 0232 > Monolithic silica r...
- 9 of 226
- Previous record
- Next record
- To hitlist
- Lutz, Mareike,1967-DMPK & Bioanalytical Chemistry, AstraZeneca R&D Mölndal, Mölndal, Sweden (author)
Monolithic silica rod liquid chromatography with ultraviolet or fluorescence detection for metabolite analysis of cytochrome P450 marker reactions
- Article/chapterEnglish2002
Publisher, publication year, extent ...
- Amsterdam :Elsevier,2002
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:hh-24120
- https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-24120URI
- https://doi.org/10.1016/S1570-0232(02)00264-7DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- In vitro cytochrome P450 assays are used in metabolism studies in support of early phases of drug discovery to investigate, e.g., metabolic stability, enzyme inhibition and induction by new chemical entities. LC-UV and LC-fluorescence are traditional analytical tools in support of such studies. However, these tools typically comprise different methods of relatively low throughput for the various metabolites of probe reactions. In recent years, LC-MS methods have been developed to increase throughput. Increased throughput can also be achieved by means of modern chromatographic tools in combination with UV and fluorescence detection. This approach is especially suitable when cytochrome P450 isoforms are investigated by means of single probe incubations. Here, an LC-UV/fluorescence system based on a monolithic porous silica column is described for the analysis of metabolites of nine cytochrome P450 marker reactions [phenacetin to paracetamol (CYP1A2), coumarin to 7-hydroxycoumarin (CYP2A6), paclitaxel to 6alpha-hydroxypaclitaxel (CYP2C8), diclofenac to 4-hydroxydiclofenac (CYP2C9), mephenytoin to 4-hydroxymephenytoin (CYP2C19), bufuralol to 1-hydroxybufuralol (CYP2D6), chlorzoxazone to 6-hydroxychlorzoxazone (CYP2E1), midazolam to 1-hydroxymidazolam (CYP3A4), and testosteron to 6beta-hydroxytestosteron (CYP3A4)]. While offering sensitivities and linear ranges comparable to previously reported methods, the set-up described here provides ease of use and increased throughput with maximum cycle times of 4.5 min. © 2002 Elsevier Science B.V. All rights reserved.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- Monolithic silica rod
- Cytochrome P450
Added entries (persons, corporate bodies, meetings, titles ...)
- Markling, Magnus E.DMPK & Bioanalytical Chemistry, AstraZeneca R&D Mölndal, Mölndal, Sweden (author)
- Masimirembwa, Collen M.DMPK & Bioanalytical Chemistry, AstraZeneca R&D Mölndal, Mölndal, Sweden (author)
- DMPK & Bioanalytical Chemistry, AstraZeneca R&D Mölndal, Mölndal, Sweden (creator_code:org_t)
Related titles
- In:Journal of chromatography. BAmsterdam : Elsevier780:2, s. 205-2151570-02321873-376X
Internet link
Find in a library
- Journal of chromatography. B (Search for host publication in LIBRIS)
To the university's database
- 9 of 226
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Lutz, Mareike, 1 ...
- Markling, Magnus ...
- Masimirembwa, Co ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Analytical Chemi ...
- Articles in the publication
- Journal of chrom ...
- By the university
- Halmstad University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
