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Somatic hybridizati...
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Waara, Sylvia,1958-Department of Plant Physiology, Uppsala University, Uppsala, Sweden
(författare)
Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis
- Artikel/kapitelEngelska1989
Förlag, utgivningsår, omfång ...
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Heidelberg :Springer Berlin/Heidelberg,1989
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:hh-30779
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https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-30779URI
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https://doi.org/10.1007/BF00292315DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Green mesophyll protoplasts of the dihaploid potato line 198∶2 (Solanum tuberosum L.) were fused with herbicide-bleached mesophyll protoplasts of the dihaploid potato line 67∶9 using a polyethylene glycol protocol. Heterokaryons were identified under a fluorescence microscope using the dual fluorescence of carboxyfluorescein-stained, herbicide-bleached protoplasts and the autofluorescence of green mesophyll protoplasts. About 20% of the protoplasts survived the fusion treatment, and the fusion frequency was 3%-4%. Unfused and fused protoplasts were mass cultured for 6 weeks after which vigorously growing calli were selected and transferred to shoot regeneration medium. Somatic hybrids were identified by a combination of five isozyme markers, and the ploidy level was determined by flow cytometry. Out of 15 calli that regenerated shoots, 6 plants derived from 2 different calli were identified as hexaploid somatic hybrids, while one morphologically deviant plant from a third callus was identified as a mixoploid that had lost some enzyme markers after 4 months of culturing.
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Tegelström, H.Department of Plant Physiology, Uppsala University, Uppsala, Sweden
(författare)
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Wallin, AnitaDepartment of Plant Physiology, Uppsala University, Uppsala, Sweden
(författare)
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Eriksson, TageDepartment of Plant Physiology, Uppsala University, Uppsala, Sweden
(författare)
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Department of Plant Physiology, Uppsala University, Uppsala, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Theoretical and Applied GeneticsHeidelberg : Springer Berlin/Heidelberg77:1, s. 49-560040-57521432-2242
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