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Effects of Differen...
Effects of Different Exercise Training Protocols on Gene Expression of Rac1 and PAK1 in Healthy Rat Fast- and Slow-Type Muscles.
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- Laine, Saara (författare)
- urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland
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- Högel, Heidi (författare)
- Turku Centre for Biotechnology, University of Turku, Åbo Akademi University, Turku, Finland; Natural Resources Institute Finland (Luke), Jokioinen, Finland
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- Ishizu, Tamiko (författare)
- Turku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland; Institute of Biomedicine, University of Turku, Turku, Finland; TuDMM Doctoral Programmes, University of Turku, Turku, Finland
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- Toivanen, Jussi (författare)
- urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland
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- Yli-Karjanmaa, Minna (författare)
- urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland
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- Grönroos, Tove J (författare)
- urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland
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- Rantala, Juha (författare)
- Misvik Biology, Turku, Finland
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- Mäkelä, Rami (författare)
- Misvik Biology, Turku, Finland
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- Hannukainen, Jarna C (författare)
- Turku PET Centre, Turku University Hospital, University of Turku, Turku, Finland
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- Kalliokoski, Kari K (författare)
- Turku PET Centre, Turku University Hospital, University of Turku, Turku, Finland
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- Heinonen, Ilkka, 1982- (författare)
- Högskolan i Halmstad,Rydberglaboratoriet för tillämpad naturvetenskap (RLAS),Exercise Biomedicine Research group
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(creator_code:org_t)
- 2020-11-19
- 2020
- Engelska.
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Ingår i: Frontiers in Physiology. - : Frontiers Media S.A.. - 1664-042X. ; 11
- Relaterad länk:
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https://doi.org/10.3...
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Purpose: Rac1 and its downstream target PAK1 are novel regulators of insulin and exercise-induced glucose uptake in skeletal muscle. However, it is not yet understood how different training intensities affect the expression of these proteins. Therefore, we studied the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on Rac1 and PAK1 expression in fast-type (gastrocnemius, GC) and slow-type (soleus, SOL) muscles in rats after HIIT and MICT swimming exercises.Methods: The mRNA expression was determined using qPCR and protein expression levels with reverse-phase protein microarray (RPPA).Results: HIIT significantly decreased Rac1 mRNA expression in GC compared to MICT (p = 0.003) and to the control group (CON) (p = 0.001). At the protein level Rac1 was increased in GC in both training groups, but only the difference between HIIT and CON was significant (p = 0.02). HIIT caused significant decrease of PAK1 mRNA expression in GC compared to MICT (p = 0.007) and to CON (p = 0.001). At the protein level, HIIT increased PAK1 expression in GC compared to MICT and CON (by ∼17%), but the difference was not statistically significant (p = 0.3, p = 0.2, respectively). There were no significant differences in the Rac1 or PAK1 expression in SOL between the groups.Conclusion: Our results indicate that HIIT, but not MICT, decreases Rac1 and PAK1 mRNA expression and increases the protein expression of especially Rac1 but only in fast-type muscle. These exercise training findings may reveal new therapeutic targets to treat patients with metabolic diseases.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- HIIT
- MICT
- PAK1
- RPPA
- Rac1
- exercise
- glucose uptake
- skeletal muscle
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Laine, Saara
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Högel, Heidi
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Ishizu, Tamiko
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Toivanen, Jussi
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Yli-Karjanmaa, M ...
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Grönroos, Tove J
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visa fler...
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Rantala, Juha
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Mäkelä, Rami
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Hannukainen, Jar ...
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Kalliokoski, Kar ...
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Heinonen, Ilkka, ...
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Högskolan i Halmstad