Search: id:"swepub:oai:DiVA.org:hh-48806" >
Evaluation of the p...
-
Corthay, AlexandreUniversity of Oslo, National Hospital, Oslo, Norway; Lund University, Lund, Sweden
(author)
Evaluation of the percentage of peripheral T cells with two different T cell receptor alpha-chains and of their potential role in autoimmunity
- Article/chapterEnglish2001
Publisher, publication year, extent ...
-
Rockville, MD :American Association of Immunologists,2001
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:hh-48806
-
https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-48806URI
-
https://doi.org/10.1006/jaut.2001.0504DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Approximately 25% of mature T cells possess two distinct cytoplasmic T cell receptor (TCR) alpha-chains, due to productive gene rearrangements of both alleles. Expression of two different alpha-chains at the cell surface is a potential risk factor for development of autoimmunity. However, it has been difficult to determine the frequency of peripheral T cells with two different alpha-chains at the surface. Our new approach is based on comparing by flow cytometry the percentage of cells that express a given Valpha-chain between wild-type mice and mice that are hemizygous for a disrupted Tcra locus (Tcra+/-) and consequently unable to express two rearranged Tcra genes. We consistently found that approximately 8% of total peripheral T cells express two surface alpha-chains. The importance of dual alpha-T cells in autoimmunity was examined in a mouse model for rheumatoid arthritis, namely collagen-induced arthritis (CIA). No significant difference was observed between Tcra+/- mice and wild-type littermates, considering arthritis incidence, day of disease onset, and maximum arthritic score. We therefore conclude that there is incomplete phenotypic allelic exclusion in TCRalpha, and that the presence of a significant number of potentially multireactive T cells does not increase the susceptibility to develop autoimmune arthritis. © 2001 Academic Press.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Nandakumar, Kutty Selva,1965-Lund University, Lund, Sweden(Swepub:hh)kutnam
(author)
-
Holmdahl, RikardLund University, Lund, Sweden
(author)
-
University of Oslo, National Hospital, Oslo, Norway; Lund University, Lund, SwedenLund University, Lund, Sweden
(creator_code:org_t)
Related titles
-
In:Journal of AutoimmunityRockville, MD : American Association of Immunologists16:4, s. 423-4290896-84111095-9157
Internet link
Find in a library
To the university's database