Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Sachinidis Agapios) > Gene networks and t...

Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells

Godoy, Patricio (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany / Department of Physiology, Faculty of Biological Sciences, University of Concepción, Chile

Schmidt-Heck, Wolfgang (författare): Leibniz Institute for Natural Product Research and Infection Biology eV-Hans-Knöll Institute, Jena, Germany

Natarajan, Karthick (författare): University of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, Germany

visa fler...

Lucendo-Villarin, Baltasar (författare): MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom

Szkolnicka, Dagmara (författare): MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom

Asplund, Annika (författare): Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden,Bioinformatik, Bioinformatics

Björquist, Petter (författare): NovaHep AB, Gothenburg, Sweden

Widera, Agata (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

Stöber, Regina (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

Campos, Gisela (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

Hammad, Seddik (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

Sachinidis, Agapios (författare): University of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, Germany

Chaudhari, Umesh (författare): University of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, Germany

Damm, Georg (författare): Charité University Medicine Berlin, Department of General-, Visceral- and Transplantation Surgery, Berlin, Germany

Weiss, Thomas S. (författare): Center for Liver Cell Research, Department of Pediatrics and Juvenile Medicine, University of Regensburg Hospital, Regensburg, Germany

Nüssler, Andreas (författare): Eberhard Karls University Tübingen, BG Trauma Center, Siegfried Weller Institut, Tübingen, Germany

Synnergren, Jane (författare): Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatics

Edlund, Karolina (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

Küppers-Munther, Barbara (författare): Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden

Hay, David C. (författare): MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom

Hengstler, Jan G. (författare): IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany

visa färre...

(creator_code:org_t)

Elsevier, 2015
2015
Engelska.
Ingår i: Journal of Hepatology. - : Elsevier. - 0168-8278 .- 1600-0641. ; 63:4, s. 934-942

Relaterad länk:: https://doi.org/10.1...; visa fler...; https://doi.org/10.1...; https://his.diva-por... (primary) (Raw object); http://www.journal-o...; https://urn.kb.se/re...; https://doi.org/10.1...; visa färre...

Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

BACKGROUND & AIMS: The differentiation of stem cells to hepatocyte-like cells (HLC) offers the perspective of unlimited supply of human hepatocytes. However, the degree of differentiation of HLC remains controversial. To obtain an unbiased characterization, we performed a transcriptomic study with HLC derived from human embryonic and induced stem cells (ESC, hiPSC) from three different laboratories.METHODS: Genome-wide gene expression profiles of ESC and HLC were compared to freshly isolated and up to 14days cultivated primary human hepatocytes. Gene networks representing successful and failed hepatocyte differentiation, and the transcription factors involved in their regulation were identified.RESULTS: Gene regulatory network analysis demonstrated that HLC represent a mixed cell type with features of liver, intestine, fibroblast and stem cells. The "unwanted" intestinal features were associated with KLF5 and CDX2 transcriptional networks. Cluster analysis identified highly correlated groups of genes associated with mature liver functions (n=1057) and downregulated proliferation associated genes (n=1562) that approach levels of primary hepatocytes. However, three further clusters containing 447, 101, and 505 genes failed to reach levels of hepatocytes. Key TF of two of these clusters include SOX11, FOXQ1, and YBX3. The third unsuccessful cluster, controlled by HNF1, CAR, FXR, and PXR, strongly overlaps with genes repressed in cultivated hepatocytes compared to freshly isolated hepatocytes, suggesting that current in vitro conditions lack stimuli required to maintain gene expression in hepatocytes, which consequently also explains a corresponding deficiency of HLC.CONCLUSIONS: The present gene regulatory network approach identifies key transcription factors which require modulation to improve HLC differentiation.

Hitta via bibliotek

Journal of Hepatology (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Cellbiologi

Artiklar i publikationen: Journal of Hepat ...

Av lärosätet: Högskolan i Skövde

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se

Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells

Ämnesord

Nyckelord

Publikations- och innehållstyp

Hitta via bibliotek

Till lärosätets databas

Hitta mer i SwePub

Sök utanför SwePub