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Transcriptional and...
Transcriptional and biochemical biomarker responses in a freshwater mussel (Anodonta anatina) under environmentally relevant Cu exposure
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- Ekelund Ugge, Gustaf Magnus Oskar (författare)
- University of Skövde,Lund University,Lunds universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Department of Biology, Lund University, Sweden,Ekologisk modellering, Ecological Modeling,Enhet akvatisk ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Division aquatic ecology,Faculty of Science
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- Jonsson, Annie (författare)
- University of Skövde,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Ekologisk modellering, Ecological Modeling
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- Olsson, Björn, 1962- (författare)
- University of Skövde,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Translationell bioinformatik, Translational Bioinformatics
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- Sjöback, Robert (författare)
- TATAA Biocenter, Gothenburg, Sweden,TATAA Biocenter AB
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- Berglund, Olof (författare)
- Lund University,Lunds universitet,Enhet akvatisk ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Division aquatic ecology,Department of Biology,Faculty of Science,Department of Biology, Lund University, Sweden
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(creator_code:org_t)
- 2020-01-13
- 2020
- Engelska.
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Ingår i: Environmental Science and Pollution Research. - : Springer Berlin/Heidelberg. - 0944-1344 .- 1614-7499. ; 27:9, s. 9999-10010
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Molecular biomarkers, like gene transcripts or enzyme activities, are potentially powerful tools for early warning assessment of pollution. However, a thorough understanding of response and baseline variation is required to distinguish actual effects from pollution. Here, we assess the freshwater mussel Anodonta anatina as a biomarker model species for freshwater ecosystems, by testing responses of six transcriptional (cat, gst, hsp70, hsp90, mt, and sod) and two biochemical (AChE and GST) biomarkers to environmentally relevant Cu water concentrations. Mussels (n = 20), collected from a stream free from point source pollution, were exposed in the laboratory, for 96 h, to Cu treatments (< 0.2 mu g/L, 0.77 +/- 0.87 mu g/L, and 6.3 +/- 5.4 mu g/L). Gills and digestive glands were extracted and analyzed for transcriptional and biochemical responses. Biological and statistical effect sizes from Cu treatments were in general small (mean log(2) fold-change <= 0.80 and Cohen's f <= 0.69, respectively), and no significant treatment effects were observed. In contrast, four out of eight biomarkers (cat, gst, hsp70, and GST) showed a significant sex:tissue interaction, and additionally one (sod) showed significant overall effects from sex. Specifically, three markers in gills (cat, mt, GST) and one in digestive gland (AChE) displayed significant sex differences, independent of treatment. Results suggest that sex or tissue effects might obscure low-magnitude biomarker responses and potential early warnings. Thus, variation in biomarker baselines and response patterns needs to be further addressed for the future use of A. anatina as a biomarker model species.
Ämnesord
- NATURVETENSKAP -- Biologi -- Annan biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Other Biological Topics (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
Nyckelord
- Bivalve
- Gene expression
- Response variability
- Sex effects
- Effect size
- RT-qPCR
- INF502 Biomarkers
- INF502 Biomarkörer
- Ekologisk modellering
- Ecological Modelling Group
- Bioinformatik
- Bioinformatics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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