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  • Granath, CarlKarolinska Institutet,Division of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden (författare)

Characterization of Laminins in Healthy Human Aortic Valves and a Modified Decellularized Rat Scaffold

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • Mary Ann Liebert,2020
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:his-19350
  • https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-19350URI
  • https://doi.org/10.1089/biores.2020.0018DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-430117URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:233376633URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • CC BY 4.0
  • Aortic valve stenosis is one of the most common cardiovascular diseases in western countries and can only be treated by replacement with a prosthetic valve. Tissue engineering is an emerging and promising treatment option, but in-depth knowledge about the microstructure of native heart valves is lacking, making the development of tissue-engineered heart valves challenging. Specifically, the basement membrane (BM) of heart valves remains incompletely characterized, and decellularization protocols that preserve BM components are necessary to advance the field. This study aims to characterize laminin isoforms expressed in healthy human aortic valves and establish a small animal decellularized aortic valve scaffold for future studies of the BM in tissue engineering. Laminin isoforms were assessed by immunohistochemistry with antibodies specific for individual alpha, beta, and gamma chains. The results indicated that LN-411, LN-421, LN-511, and LN-521 are expressed in human aortic valves (n = 3), forming a continuous monolayer in the endothelial BM, whereas sparsely found in the interstitium. Similar results were seen in rat aortic valves (n = 3). Retention of laminin and other BM components, concomitantly with effective removal of cells and residual DNA, was achieved through 3 h exposure to 1% sodium dodecyl sulfate and 30 min exposure to 1% Triton X-100, followed by nuclease processing in rat aortic valves (n = 3). Our results provide crucial data on the microenvironment of valvular cells relevant for research in both tissue engineering and heart valve biology. We also describe a decellularized rat aortic valve scaffold useful for mechanistic studies on the role of the BM in heart valve regeneration.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Noren, HunterCell Therapy Institute, Dr. Kiran C. Patel College of Allopathic Medicine, Nova Southeastern University, Davie, Florida, USA,Nova Southeastern Univ, Dr Kiran C Patel Coll Allopath Med, Cell Therapy Inst, Davie, FL USA. (författare)
  • Björck, HannaKarolinska Institutet,Cardiovascular Medicine Unit, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden (författare)
  • Simon, NancyCardiovascular Medicine Unit, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden,Karolinska Univ Hosp, Ctr Mol Med, Karolinska Inst, Cardiovasc Med Unit,Dept Med, Stockholm, Sweden. (författare)
  • Olesen, KimUppsala universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Division of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden / Department of Chemistry, Ångström Laboratory, Uppsala University, Sweden,Infektionsbiologi, Infection Biology,Polymerkemi,Karolinska Inst, Dept Mol Med & Surg, Div Cardiothorac Surg, Stockholm, Sweden.;Univ Skövde, Dept Biosci, Skövde, Sweden.(Swepub:uu)kimol250 (författare)
  • Rodin, SergeyUppsala universitet,Thoraxkirurgi,Karolinska Inst, Dept Med Biochem & Biophys, Chem 1, Stockholm, Sweden.,Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden / Division of Cardiothoracic Surgery and Anesthesiology, Department of Surgical Sciences, Uppsala University, Akademiska University Hospital, Uppsala, Sweden(Swepub:uu)serro142 (författare)
  • Grinnemo, Karl-HenrikUppsala universitet,Thoraxkirurgi,Karolinska Inst, Dept Mol Med & Surg, Div Cardiothorac Surg, Stockholm, Sweden.,Division of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden / Division of Cardiothoracic Surgery and Anesthesiology, Department of Surgical Sciences, Uppsala University, Akademiska University Hospital, Uppsala, Sweden(Swepub:uu)kargr698 (författare)
  • Österholm, CeciliaKarolinska Institutet,Division of Clinical Genetics, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden (författare)
  • Karolinska InstitutetDivision of Cardiothoracic Surgery, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:BioResearch Open Access: Mary Ann Liebert9:1, s. 269-2782164-78442164-7860

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