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Differential expres...
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Diaz Cruz, Maria AraceliJönköping University,HHJ. Biomedicinsk plattform,Research School of Health and Welfare, School of Health and Welfare, University of Jönköping, Sweden
(författare)
Differential expression of protein disulfide-isomerase A3 isoforms, PDIA3 and PDIA3N, in human prostate cancer cell lines representing different stages of prostate cancer
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
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2021-03-24
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Springer,2021
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:his-19611
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https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-19611URI
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https://doi.org/10.1007/s11033-021-06277-1DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-52111URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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CC BY 4.0
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Prostate cancer (PCa) is a highly heterogeneous and unpredictable progressive disease. Sensitivity of PCa cells to androgens play a central role in tumor aggressiveness but biomarkers with high sensitivity and specificity that follow the progression of the disease has not yet been verified. The vitamin D endocrine system and its receptors, the Vitamin D Receptor (VDR) and the Protein Disulfide-Isomerase A3 (PDIA3), are related to anti-tumoral effects as well as carcinogenesis and have therefore been suggested as potential candidates for the prevention and therapy of several cancer forms, including PCa. In this study, we evaluated the mRNA expression of VDR and PDIA3 involved in vitamin D signaling in cell lines representing different stages of PCa (PNT2, P4E6, LNCaP, DU145 and PC3). This study further aimed to evaluate vitamin D receptors and their isoforms as potential markers for clinical diagnosis of PCa. A novel transcript isoform of PDIA3 (PDIA3N) was identified and found to be expressed in all PCa cell lines analyzed. Androgen-independent cell lines showed a higher mRNA expression ratio between PDIA3N/PDIA3 contrary to androgen-dependent cell lines that showed a lower mRNA expression ratio between PDIA3N/PDIA3. The structure of PDIA3N differed from PDIA3. PDIA3N was found to be a N-truncated isoform of PDIA3 and differences in protein structure suggests an altered protein function i.e. cell location, thioredoxin activity and affinity for 1,25(OH)2D3. Collectively, PDIA3 transcript isoforms, the ratio between PDIA3N/PDIA3 and especially PDIA3N, are proposed as candidate markers for future studies with different stages of PCa progression.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Karlsson, SandraJönköping University,HHJ, Avdelningen för naturvetenskap och biomedicin,HHJ. Biomedicinsk plattform,Department of Natural Science and Biomedicine, School of Health and Welfare, University of Jönköping, Sweden(Swepub:hj)karsan
(författare)
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Szekeres, FerencHögskolan i Skövde,Institutionen för hälsovetenskaper,Forskningsmiljön hälsa, hållbarhet och digitalisering,Translationell medicin TRIM, Translational Medicine,Department of Biomedicine, School of Health Sciences, University of Skövde, Skövde, Sweden(Swepub:his)szef
(författare)
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Faresjö, MariaJönköping University,HHJ, Avdelningen för naturvetenskap och biomedicin,HHJ. Biomedicinsk plattform,Department of Natural Science and Biomedicine, School of Health and Welfare, University of Jönköping, Sweden(Swepub:hj)farm
(författare)
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Lund, DanJönköping University,HHJ, Avdelningen för naturvetenskap och biomedicin,HHJ. Biomedicinsk plattform,Department of Natural Science and Biomedicine, School of Health and Welfare, University of Jönköping, Sweden(Swepub:hj)lundda
(författare)
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Larsson, DennisSahlgrenska University Hospital, Gothia Forum - for Clinical Research, Gothenburg, Sweden(Swepub:his)lars
(författare)
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Jönköping UniversityHHJ. Biomedicinsk plattform
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Molecular Biology Reports: Springer48:3, s. 2429-24360301-48511573-4978
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