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Exposing kinetic di...
Exposing kinetic disparities between inflammasome readouts using time-resolved analysis
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- Herring, Matthew (författare)
- Örebro universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Sweden ; Inflammatory Response and Infection Susceptibility Centre (iRiSC), Örebro University, Sweden,Infection Biology,Institutionen för medicinska vetenskaper,School of Bioscience, Systems Biology Research Centre, University of Skövde, Skövde, Sweden
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- Persson, Alexander, 1978- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
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- Potter, Ryan (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,School of Bioscience, Systems Biology Research Centre, University of Skövde, Skövde, Sweden; Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden,Infection Biology
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- Karlsson, Roger (författare)
- Nanoxis Consulting AB, Göteborg, Sweden; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Göteborg, Sweden
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- Särndahl, Eva, 1963- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Inflammatory Response and Infection Susceptibility Centre (iRiSC)
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- Ejdebäck, Mikael, 1969- (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Infection Biology,School of Bioscience, Systems Biology Research Centre, University of Skövde, Skövde, Sweden
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(creator_code:org_t)
- Elsevier, 2024
- 2024
- Engelska.
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Ingår i: Heliyon. - : Elsevier. - 2405-8440. ; 10:11
- Relaterad länk:
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https://doi.org/10.1...
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https://his.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The NLRP3 inflammasome is an intracellular multiprotein complex described to be involved in both an effective host response to infectious agents and various diseases. Investigation into the NLRP3 inflammasome has been extensive in the past two decades, and often revolves around the analysis of a few specific readouts, including ASC-speck formation, caspase-1 cleavage or activation, and cleavage and release of IL-1β and/or IL-18. Quantification of these readouts is commonly undertaken as an endpoint analysis, where the presence of each positive outcome is assessed independently of the others. In this study, we apply time-resolved analysis of a human macrophage model (differentiated THP-1-ASC-GFP cells) to commonly accessible methods. This approach yields the additional quantifiable metrics time-resolved absolute change and acceleration, allowing comparisons between readouts. Using this methodological approach, we reveal (potential) discrepancies between inflammasome-related readouts that otherwise might go undiscovered. The study highlights the importance of time-resolved data in general and may be further extended as well as incorporated into other areas of research.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Medicinsk bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- ASC-Specks
- Cell response
- Cytokines
- Human macrophages
- LDH leakage
- Live-cell imaging
- NLRP3 inflammasome
- THP-1 cells
- Infection Biology
- Infektionsbiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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