Knockdown of peroxisome proliferator-activated receptor-β induces less differentiation and enhances cell-fibronectinadhesion of colon cancer cells

The role of peroxisome proliferator-activated receptor-/ (PPAR-/) in the pathogenesis of colon cancer remains highly controversial. This study specifically silenced the PPAR- expression in three colon cancer cell lines with different metastatic potentials. Although PPAR-knockdown resulted in more malignant morphological changes, bigger colony sizes and lower carcinoembryonic antigen (CEA) secretion, and enhanced the cell-fibronectin adhesion, cell invasion and migration were unaffected. These effects were stronger in poorly metastatic cell lines compared with highly metastatic ones. Simultaneously, PPAR-knockdown decreased the mRNAs encoding adipocyte differentiation-related protein and liver fatty acid binding protein, and increased the mRNA of ILK, whereas the mRNAs encoding integrin-1 and angiopoietin-like 4 were unchanged. Using immunohistochemistry, we determined that the intensity of PPAR- expression was stronger in rectal cancers with better differentiation than in those with poor differentiation, and was stronger in early-stage tumors than in advanced ones. Together, these findings consistently indicate that PPAR- may facilitate differentiation and inhibit the cell-fibronectin adhesion of colon cancer, having a role as an inhibitor in the carcinogenesis and progression of colorectal cancer. Interestingly PPAR- seems to have a more important role in poorly metastatic cells than in highly metastatic ones.

Olsson, B.Linköping University (författare)
Pfeifer, D.Linköping University (författare)
Jönsson, J.-I.Linköping University (författare)
Zhou, Z.-G.Sichuan University (författare)
Jiang, X.Sichuan University (författare)
Fredriksson, B.-A.Linköping University (författare)
Zhang, HongHögskolan i Skövde,Institutionen för vård och natur,Forskningscentrum för Systembiologi(Swepub:his)zhah (författare)
Sun, X.-F.Linköping University (författare)
Linköping UniversitySichuan University (creator_code:org_t)

Av författaren/redakt...: Yang, L.; Olsson, B.; Pfeifer, D.; Jönsson, J.-I.; Zhou, Z.-G.; Jiang, X.; visa fler...; Fredriksson, B.- ...; Zhang, Hong; Sun, X.-F.; visa färre...

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