Modeling Competitive Adsorption Isotherms in Gradient Elution Nonlinear Reversed Phase Liquid Chromatography.

• Several important studies – based on firm theory ground - have been made on the different parameters of importance in chromatographic process optimizations during the recent years. In this presentation we take an holistic view by investigating i) the relative importance of the operational parameters and column properties and ii) how these effect the optimal column design. For optimization we used an advanced global response surface method combined with local gradient methods. As a model system we used the practical problem of purification of one or both optical isomers of a racemate. In the first part, we investigated the relative importance of stationary phase characteristics such as: (i) the retention factor, (ii) the selectivity, (iii) the saturation capacity (iv), the efficiency and (v) the maximum allowed operational pressure. In the second part we investigated how the optimal column design for a preparative separation problem is affected by column properties, such as particle size, and optimization constraints, such as required yield. The investigation showed – in contrast to what is generally believed – that the saturation capacity of the stationary phase is of minor importance, instead the maximum allowed operational pressure is one of the most important parameter. Moreover, smaller size packing materials always shows much lower solvent consumption. This is a great environmental and economical advantage of using smaller packing materials. Rules of thumbs, derived from the holistic optimizations, will be given for industrial preparative settings in the batch mode.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

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