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Genome-wide profilin...
Genome-wide profiling of Populus small RNAs
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- Klevebring, Daniel, 1981- (författare)
- KTH,Genteknologi,School of Biotechnology, Division of Gene Technology, AlbaNova University Center, Royal Institute of Technology, 106 91 Stockholm, Sweden
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- Street, Nathaniel R, 1979- (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Fahlgren, Noah (författare)
- Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331, USA
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- Kasschau, Kristin D. (författare)
- Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331, USA
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- Carrington, James C. (författare)
- Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331, USA
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- Lundeberg, Joakim (författare)
- KTH,Genteknologi,School of Biotechnology, Division of Gene Technology, AlbaNova University Center, Royal Institute of Technology, 106 91 Stockholm, Sweden
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- Jansson, Stefan, 1959- (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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(creator_code:org_t)
- 2009-12-20
- 2009
- Engelska.
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 10, s. Article number 620-
- Relaterad länk:
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https://bmcgenomics....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Short RNAs, and in particular microRNAs, are important regulators of gene expression both within defined regulatory pathways and at the epigenetic scale. We investigated the short RNA (sRNA) population (18-24 nt) of the transcriptome of green leaves from the sequenced Populus trichocarpa using a concatenation strategy in combination with 454 sequencing. Results: The most abundant size class of sRNAs were 24 nt and these were generally associated with a number of classes of retrotransposons and repetitive elements. Some repetitive elements were also associated with 22 nt RNAs. We identified an sRNA hot-spot on chromosome 19, overlapping a region containing both the sex-determining loci and a major cluster of NBS-LRR genes. A number of phased siRNA loci were identified, a subset of which are predicted to target PPR and NBS-LRR disease resistance genes, classes of genes that have been significantly expanded in Populus. Additional loci enriched for sRNA production were identified. We identified 15 novel predicted microRNAs (miRNAs), including miRNA∗ sequences, and identified a novel locus that may encode a dual miRNA or a miRNA and short interfering RNAs (siRNAs). Conclusions: The short RNA population of P. trichocarpa is at least as complex as that of Arabidopsis. We provide a first genome-wide view of short RNA production for P. trichocarpa and identify new, non-conserved miRNAs.
Ämnesord
- NATURVETENSKAP -- Biologi -- Annan biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Other Biological Topics (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- stress-responsive micrornas; dna-methylation; arabidopsis-thaliana; sirna biogenesis; repetitive elements; mirna genes; trichocarpa; plants; database; expression
- Functional genomics
- Funktionsgenomik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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