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Live cell imaging i...
Live cell imaging in a micro-array of acoustic traps facilitates quantification of natural killer cell heterogeneity
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- Christakou, Athanasia E. (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Ohlin, Mathias (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Vanherberghen, Bruno (author)
- KTH,Tillämpad fysik
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- Khorshidi, Mohammad Ali (author)
- KTH,Tillämpad fysik
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- Kadri, Nadir (author)
- Karolinska Institutet
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- Frisk, Thomas (author)
- KTH,Cellens fysik
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- Wiklund, Martin (author)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Önfelt, Björn (author)
- Karolinska Institutet,KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2013-02-22
- 2013
- English.
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In: Integrative Biology. - : Oxford University Press (OUP). - 1757-9694 .- 1757-9708. ; 5:4, s. 712-719
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
Close
- Natural killer (NK) cells kill virus-infected or cancer cells through the release of cytotoxic granules into a tight intercellular contact. NK cell populations comprise individual cells with varying sensitivity to distinct input signals, leading to disparate responses. To resolve this NK cell heterogeneity, we have designed a novel assay based on ultrasound-assisted cell-cell aggregation in a multiwell chip allowing high-resolution time-lapse imaging of one hundred NK-target cell interactions in parallel. Studying human NK cells' ability to kill MHC class I deficient tumor cells, we show that approximately two thirds of the NK cells display cytotoxicity, with some NK cells being particularly active, killing up to six target cells during the assay. We also report that simultaneous interaction with several susceptible target cells increases the cytotoxic responsiveness of NK cells, which could be coupled to a previously unknown regulatory mechanism with implications for NK-mediated tumor elimination.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Nk Cells
- Education
- Cytotoxicity
- Lymphocytes
- Secretion
Publication and Content Type
- ref (subject category)
- art (subject category)
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