Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy

Sökning: L773:1422 0067 > Electrostatic Inter...

QC 20100617
Electrostatic interactions between dielectric surfaces and different fluorophoresused in ultrasensitive fluorescence microscopy are investigated using objective-based TotalInternal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacialdynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein,zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengthsat physiological pH. As analyzed by means of the amplitude and time-evolution of theautocorrelation function, the fluorescent molecules experience electrostatic attraction orrepulsion at the glass surface depending on their charges. Influences of the electrostaticinteractions are also monitored through the triplet-state population and triplet relaxationtime, including the amount of detected fluorescence or the count-rate-per-moleculeparameter. These TIR-FCS results provide an increased understanding of how fluorophoresare influenced by the microenvironment of a glass surface, and show a promising approachfor characterizing electrostatic interactions at interfaces.

Hassler, KaiKTH,Experimentell biomolekylär fysik(Swepub:kth)u1jcyp94 (författare)
Chmyrov, Andriy,1979-KTH,Experimentell biomolekylär fysik(Swepub:kth)u1lkaz3k (författare)
Widengren, JerkerKTH,Experimentell biomolekylär fysik(Swepub:kth)u1i3g09c (författare)
KTHExperimentell biomolekylär fysik (creator_code:org_t)

Ingår i:International Journal of Molecular Sciences: MDPI11:2, s. 368-4061661-65961422-0067

Av författaren/redakt...: Blom, Hans; Hassler, Kai; Chmyrov, Andriy, ...; Widengren, Jerke ...

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