Antibody based plasma protein profiling

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Qundos, Ulrika,1983-KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab (författare)

Antibody based plasma protein profiling

BokEngelska2013

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Stockholm :KTH Royal Institute of Technology,2013
viii, 68 s.
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:kth-126270
ISBN:9789175018294
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-126270URI

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Språk:engelska
Sammanfattning på:engelska

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Serie

Trita-BIO-Report,1654-2312 ;2013:12

Anmärkningar

QC 20130821
This thesis is about protein profiling in serum and plasma using antibody suspension bead arrays for the analysis of biobanked samples and in the context of prostate cancer biomarker discovery. The influence of sample preparation methods on antibody based protein profiles were investigated (Papers I-III) and a prostate cancer candidate biomarker identified and verified (Papers III-V). Furthermore, a perspective on the research area affinity proteomics and its’ employment in biomarker discovery, for improved understanding and potentially improved disease diagnosis, is provided.Paper I presents the results of a comparative plasma and serum protein profiling study, with a targeted biomarker discovery approach in the context of metabolic syndrome. The study yielded a higher number of significant findings and a low experimental variability in blood samples prepared as plasma. Paper II investigated the effects from post-centrifugation delays at different temperatures prior sample storage of serum and plasma samples. Minor effects were found on the detected levels of more than 300 predicted or known plasma proteins. In Paper III, the detectability of proteins in plasma was explored by exposing samples to different pre-analytical heat treatments, prior target capture. Heat induced epitope retrieval was observed for approximately half of the targeted proteins, and resulted in the discovery of different candidate markers for prostate cancer. Several antibodies towards the prostate cancer candidate biomarker CNDP1 were generated, epitope mapped and evaluated in a bead based sandwich immunoassay, as presented in Papers IV and V. Furthermore, the developed sandwich immunoassay targeting multiple distinct CNDP1 epitopes in more than 1000 samples, confirmed the association of CNDP1 levels to aggres- sive prostate cancer and more specifically to prostate cancer patients with regional lymph node metastasis (Paper V).As an outcome of the present investigations and in parallel to studies within the Biobank profiling research group, valuable lessons from study design and multiplex antibody analysis of plasma within biomarker discovery to experimental, technical and biological verifications have been collected.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
protein profiling
plasma
antibody
affinity proteomics
biomark- ers
multiplex
assay development

Biuppslag (personer, institutioner, konferenser, titlar ...)

Schwenk, Jochen M.,Assoc. Prof.KTH,Proteomik och nanobioteknologi(Swepub:kth)u1h7wtme (preses)
Nilsson, Peter,Prof.KTH,Proteomik och nanobioteknologi(Swepub:kth)u1ws88sk (preses)
Uhlén, Mathias,Prof.KTH,Proteomik och nanobioteknologi(Swepub:kth)u1dulvmw (preses)
Korf, Ulrike,Dr.Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ) (opponent)
KTHProteomik och nanobioteknologi (creator_code:org_t)

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Av författaren/redakt...: Qundos, Ulrika, ...; Schwenk, Jochen ...; Nilsson, Peter, ...; Uhlén, Mathias, ...; Korf, Ulrike, Dr ...

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