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Evaluation of in vi...
Evaluation of in vitro testing strategies for hazard assessment of the skin sensitization potential of “real-life” mixtures : The case of henna-based hair-colouring products containing p-phenylenediamine
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- de Ávila, Renato Ivan (författare)
- Federal University of Goiás
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- Veloso, Danillo F.M.C. (författare)
- Federal University of Goiás
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- Teixeira, Gabriel C. (författare)
- Federal University of Goiás
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- Rodrigues, Thaisângela L. (författare)
- Federal University of Goiás
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- Lindberg, Tim (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Lindstedt, Malin (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Fonseca, Simone G. (författare)
- Federal University of Goiás
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- Lima, Eliana M. (författare)
- Federal University of Goiás
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- Valadares, Marize C. (författare)
- Federal University of Goiás
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(creator_code:org_t)
- 2019-06-20
- 2019
- Engelska.
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Ingår i: Contact Dermatitis. - : Wiley. - 0105-1873 .- 1600-0536. ; 81:3, s. 194-209
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Allergic contact dermatitis caused by henna-based hair-colouring products has been associated with adulteration of henna with p-phenylenediamine (PPD). Objectives: To develop a testing approach based on in vitro techniques that address key events within the skin sensitization adverse outcome pathway in order to evaluate the allergenic potential of hair-colouring products. Methods: The following in vitro assays were used to test the sensitizing capacity of hair dye ingredients: the micro-direct peptide reactivity assay (mDPRA); the HaCaT keratinocyte-associated interleukin (IL)-18 assay; the U937 cell line activation test (U-SENS)/IL-8 levels; the blood monocyte-derived dendritic cell test; and genomic allergen rapid detection (GARD skin). Those techniques with better human concordance were selected to evaluate the allergenic potential of 10 hair-colouring products. Results: In contrast to the information on the label, chromatographic analyses identified PPD in all products. The main henna biomarker, lawsone, was not detected in one of the 10 products. Among the techniques evaluated by testing hair dye ingredients, the mDPRA, the IL-18 assay, GARD skin and the U-SENS correlated better with human classification (concordances of 91.7%-100%) and were superior to the animal testing (concordance of 78.5%). Thus, these assays were used to evaluate hair-colouring products, which were classified as skin sensitizers by the use of different two-of-three approaches. Conclusions: Our findings highlight the toxicological consequences of, and risks associated with, the undisclosed use of PPD in henna-based “natural” “real-life” products.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- allergic contact dermatitis
- alternative methods
- cosmetics
- hair dyes
- mixtures
- skin sensitization
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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