Multicolor Fluorescence Nanoscopy by Photobleaching: Concept Verification and its Application to Resolve Selective Storage of Proteins in Platelets

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:DiVA.org:kth-141008" > Multicolor Fluoresc...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Rönnlund, Daniel,1984-KTH,Experimentell biomolekylär fysik (author)

Multicolor Fluorescence Nanoscopy by Photobleaching : Concept Verification and its Application to Resolve Selective Storage of Proteins in Platelets

Article/chapterEnglish2014

Publisher, publication year, extent ...

2014-04-24
American Chemical Society (ACS),2014
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:kth-141008
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-141008URI
https://doi.org/10.1021/nn406113mDOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:129082602URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Updated from "Submitted" to "Published". QC 20140630
Fluorescence nanoscopy provides means to discernthe finer details of protein localization and interaction in cells by offeringan order of magnitude higher resolution than conventional optical imagingtechniques. However, these super resolution techniques put higher demands onthe optical system as well as on the fluorescent probes, making multicolorfluorescence nanoscopy a challenging task. Here we present a new and simpleprocedure which exploits the photostability and excitation spectra of dyes toincrease the number of simultaneous recordable targets in STED nanoscopy. Weuse this procedure to demonstrate four color STED imaging of platelets with ≤40 nm resolution and low crosstalk. Platelets can selectively store, sequesterand release a multitude of different proteins, and in a manner specific fordifferent physiological and disease states. By applying multicolor nanoscopy tostudy platelets, we can achieve spatial mapping of the protein organizationwith a high resolution, for multiple proteins at the same time and in the samecell. This provides a means to identify specific platelet activation states fordiagnostic purposes and to understand the underlying protein storage andrelease mechanisms. We studied the organization of the pro- and anti-angiogenicproteins VEGF and PF-4 together with fibrinogen and filamentous actin, andfound distinct features in their respective protein localization. Further,colocalization analysis revealed only minor overlap between the proteins VEGFand PF-4 indicating that they have separate storage and release mechanisms,corresponding well with their opposite rules as pro- and anti-angiogenicproteins, respectively.

Subject headings and genre

NATURVETENSKAP Fysik hsv//swe
NATURAL SCIENCES Physical Sciences hsv//eng
multicolor
super resolution microscopy
photobleaching
STED
platelets
thrombocytes
α-granules
Biological Physics
Biologisk fysik

Added entries (persons, corporate bodies, meetings, titles ...)

Xu, LeiKTH,Experimentell biomolekylär fysik(Swepub:kth)u1zr7k1u (author)
Perols, AnnaKTH,Proteinteknologi(Swepub:kth)u11lpgnl (author)
Eriksson Karlström, AmelieKTH,Proteinteknologi(Swepub:kth)u10x6l4n (author)
Auer, GertKarolinska Institutet (author)
Widengren, JerkerKTH,Experimentell biomolekylär fysik(Swepub:kth)u1i3g09c (author)
Gad, AKBKarolinska Institutet (author)
KTHExperimentell biomolekylär fysik (creator_code:org_t)

Related titles

In:ACS Nano: American Chemical Society (ACS)8:5, s. 4358-43651936-08511936-086X

Internet link

Find in a library

ACS Nano (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Rönnlund, Daniel ...; Xu, Lei; Perols, Anna; Eriksson Karlstr ...; Auer, Gert; Widengren, Jerke ...; show more...; Gad, AKB; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...

Articles in the publication: ACS Nano

By the university: Royal Institute of Technology; Karolinska Institutet

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se