Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides

• A novel class of nonionic, carbohydrate-based surfactants has been synthesized from the plant polysaccharide xyloglucan. Enzymatic hydrolysis of xyloglucan yielded a series of well-defined, highly branched oligosaccharides that, following reductive amination, were readily conjugated with fatty acids bearing C-8 to C-18 chains under mild conditions. The critical micelle concentration, determined by tensiometry and dye-inclusion measurements, showed a typical dependence on acyl chain length and was sensitive to the degree of galactosylation of the head group. Several compounds from this new group of surfactants, especially those with C-14 and C-16 chains, were useful for the extraction of membrane-bound enzyme markers from different plant cell compartments in catalytically active form.

Nyckelord

membrane protein extraction

reductive amination

surfactant

xyloglucan

xyloglucan oligosaccharides

sugar-based surfactants

beta-glucan synthesis

plant-cell walls

in-vitro

structural-analysis

callose synthase

interfacial behavior

product entrapment

plasma-membrane

cotton fiber

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)