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A chemically modifi...
A chemically modified α-amylase with a molten-globule state has entropically driven enhanced thermal stability
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-08-09
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Oxford University Press (OUP),2010
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-149584
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-149584URI
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https://doi.org/10.1093/protein/gzq051DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20140826
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The thermostability properties of TAA were investigated by chemically modifying carboxyl groups on the surface of the enzyme with AMEs. The TAAMOD exhibited a 200 improvement in starch-hydrolyzing productivity at 60°C. By studying the kinetic, thermodynamic and biophysical properties, we found that TAAMOD had formed a thermostable, MG state, in which the unfolding of the tertiary structure preceded that of the secondary structure by at least 20°C. The X-ray crystal structure of TAAMOD revealed no new permanent interactions (electrostatic or other) resulting from the modification. By deriving thermodynamic activation parameters of TAAMOD, we rationalised that thermostabilisation have been caused by a decrease in the entropy of the transition state, rather than being enthalpically driven. Far-UV CD shows that the origin of decreased entropy may have arisen from a higher helical content of TAAMOD. This study provides new insight into the intriguing properties of an MG state resulting from the chemical modification of TAA.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Poljak, A.
(author)
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De Francisci, D.
(author)
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Guerriero, GeaUniversity of New South Wales, Sydney, Australia(Swepub:kth)u1p3u22n
(author)
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Pilak, O.
(author)
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Burg, D.
(author)
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Raftery, M. J.
(author)
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Parkin, D. M.
(author)
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Trewhella, J.
(author)
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Cavicchioli, R.
(author)
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University of New South Wales, Sydney, Australia
(creator_code:org_t)
Related titles
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In:Protein Engineering Design & Selection: Oxford University Press (OUP)23:10, s. 769-7801741-01261741-0134
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- By the author/editor
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Siddiqui, K. S.
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Poljak, A.
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De Francisci, D.
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Guerriero, Gea
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Pilak, O.
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Burg, D.
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show more...
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Raftery, M. J.
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Parkin, D. M.
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Trewhella, J.
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Cavicchioli, R.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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Protein Engineer ...
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Royal Institute of Technology