Sökning: L773:0009 8981 OR L773:1873 3492 >
Pyrosequencing :
-
Ahmadian, AfshinKTH,Genteknologi
(författare)
Pyrosequencing : History, biochemistry and future
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
-
Elsevier BV,2006
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:kth-15369
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-15369URI
-
https://doi.org/10.1016/j.cccn.2005.04.038DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:for swepub-publicationtype
Anmärkningar
-
QC 20100525
-
Background: Pyrosequencing is a DNA sequencing technology based on the sequencing-by-synthesis principle. Methods: The technique is built on a 4-enzyme real-time monitoring of DNA synthesis by bioluminescence using a cascade that upon nucleotide incorporation ends in a detectable light signal (bioluminescence). The detection system is based on the pyrophosphate released when a nucleotide is introduced in the DNA-strand. Thereby, the signal can be quantitatively connected to the number of bases added. Currently, the technique is limited to analysis of short DNA sequences exemplified by single-nucleotide polymorphism analysis and genotyping. Mutation detection and single-nucleotide polymorphisin genotyping require screening of large samples of materials and therefore the importance of high-throughput DNA analysis techniques is significant. In order to expand the field for pyrosequencing, the read length needs to be improved. Conclusions: Th pyrosequencing system is based on an enzymatic system. There are different current and future applications of this technique.
Ämnesord och genrebeteckningar
-
pyrosequencing
-
real-time monitoring
-
SNP
-
bioluminscence
-
dna-binding-protein
-
polymerase-chain-reaction
-
single-nucleotide polymorphisms
-
micromachined filter-chamber
-
allele-specific extension
-
real-time pyrophosphate
-
tumor-suppressor gene
-
i reaction pathway
-
firefly luciferase
-
escherichia-coli
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Ehn, M.
(författare)
-
Hober, SophiaKTH,Proteomik(Swepub:kth)u11qqzc1
(författare)
-
KTHGenteknologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Clinica Chimica Acta: Elsevier BV363:02-jan, s. 83-940009-89811873-3492
Internetlänk
Hitta via bibliotek
Till lärosätets databas