Sökning: WFRF:(Olofsson Sigvard 1948) >
Decreased immune re...
Decreased immune reactivity towards a knobless, affibody-targeted adenovirus type 5 vector
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- Myhre, Susanna, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Henning, Petra, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Granio, O. (författare)
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- Tylö, Anne-Sofie, 1946 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Nygren, Per-Åke (författare)
- KTH,Molekylär Bioteknologi
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- Olofsson, Sigvard, 1948 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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Boulanger, P. (författare)
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Lindholm, L. (författare)
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Hong, S. S. (författare)
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(creator_code:org_t)
- 2006-10-12
- 2007
- Engelska.
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Ingår i: Gene Therapy. - : Springer Science and Business Media LLC. - 0969-7128 .- 1476-5462. ; 14:4, s. 376-381
- Relaterad länk:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- In this study, a prototype Adenovirus type 5 (Ad5) vector deleted of the fiber knob domain and carrying an Affibody molecule as the targeting ligand showed decreased susceptibility to human pre-existing antibodies. This vector, Ad5/R7-Z(taq)Z(taq), has short fibers carrying seven shaft repeats, a non-native trimerization signal and an affibody molecule (Z(taq)) reactive to Taq polymerase. Ad5/R7-Z(taq)Z(taq) could be specifically targeted to 293 cells stably expressing membrane-bound anti-Z(taq) idiotypic affibody called Z(ztaq) (293Z(ztaq)). Sera from 50 blood donors were analyzed for neutralization activity (NA) against the parental Ad5/Fiwt vector and knobless Ad5/R7-Z(taq)Z(taq) on 293Z(ztaq) cells. Twenty-three sera had NA titers (>= 1:64) against Ad5/Fiwt (46%) and only two against Ad5/R7-Z(taq)Z(taq) (4%). Characterization of sera with NA titers showed that the knob domain is one of the targets of the antibodies. Neutralization assays using sera pre-adsorbed on knob and hexon proteins showed that the NA of the sera was carried mainly by anti-knob and anti-hexon antibodies, but in certain sera the anti-hexon antibodies represent the major population of the neutralizing antibodies (NAbs). Our results suggested that a combination of knob deletion and hexon switching could be an effective strategy for Ad vectors to better evade the anti-Ad NAbs.
Nyckelord
- adenovirus
- virus neutralization
- neutralizing antibodies
- hexon
- fiber
- penton base
- knob
- staphylococcal protein-a
- neutralizing antibodies
- recombinant adenovirus
- virus
- gene
- humans
- determinants
- mechanism
- libraries
- receptor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Myhre, Susanna, ...
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Henning, Petra, ...
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Granio, O.
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Tylö, Anne-Sofie ...
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Nygren, Per-Åke
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Olofsson, Sigvar ...
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visa fler...
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Boulanger, P.
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Lindholm, L.
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Hong, S. S.
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- Artiklar i publikationen
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Gene Therapy
- Av lärosätet
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Kungliga Tekniska Högskolan
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Göteborgs universitet