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Sökning: WFRF:(Al Khalili Szigyarto Cristina) > (2005-2009) > Expression and subc...

  • Patrakka, Jaakko (författare)

Expression and subcellular distribution of novel glomerulus-associated proteins dendrin, ehd3, sh2d4a, plekhh2, and 2310066E14Rik

  • Artikel/kapitelEngelska2007

Förlag, utgivningsår, omfång ...

  • Ovid Technologies (Wolters Kluwer Health),2007
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-16572
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-16572URI
  • https://doi.org/10.1681/asn.2006060675DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20100525
  • The glomerular capillary tuft is a highly specialized microcapillary that is dedicated to function as a sophisticated molecular sieve. The glomerulus filter has a unique molecular composition, and several essential glomerular proteins are expressed in the kidney exclusively by glomerular podocytes. A catalog of > 300 glomerulus-upregulated transcripts that were identified using expressed sequence tag profiling and microarray analysis was published recently. This study characterized the expression profile of five glomerulus-upregulated transcripts/proteins (ehd3, dendrin, sh2d4a, plekhh2, and 2310066E14Rik) in detail. The expression pattern of these novel glomerular transcripts in various mouse tissues was studied using reverse transcriptase-PCR, Northern blotting, and in situ hybridization. For studying the distribution of corresponding proteins, polyclonal antibodies were raised against the gene products, and Western blotting, immunofluorescence, and immunoelectron microscopic analyses were performed. Remarkably, it was discovered that all five transcripts/proteins were expressed in the kidney exclusively by glomerular cells. Ehd3 was expressed only by glomerular endothelial cells. Importantly, ehd3 is the first gene ever shown to be expressed exclusively by glomerular endothelial cells and not by other endothelial cells in the kidney. Dendrin, sh2d4a, plekhh2, and 2310066E14Rik, however, were transcribed solely by podocytes. With the use of polyclonal antibodies, dendrin, sh2d4a, and plekhh2 proteins were localized to the slit diaphragm and the foot process, whereas 2310066E14Rik protein was localized to the podocyte major processes and cell body. This study provides fresh insights into glomerular biology and uncovers new possibilities to explore the role of these novel proteins in the glomerular physiology and pathology.

Ämnesord och genrebeteckningar

  • congenital nephrotic syndrome
  • pleckstrin homology domains
  • mice lacking
  • perinatal lethality
  • messenger-rna
  • nephrin
  • actin
  • podocytes
  • kidney
  • membrane

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Xiao, Zhijie (författare)
  • Nukui, Masatoshi (författare)
  • Takemoto, Minoru (författare)
  • He, Liqun (författare)
  • Oddsson, Asmundur (författare)
  • Perisic, Ljubica (författare)
  • Kaukinen, Anne (författare)
  • Al-Khalili Szigyarto, CristinaKTH,Proteomik(Swepub:kth)u1c02mh9 (författare)
  • Uhlén, MathiasKTH,Proteomik(Swepub:kth)u1dulvmw (författare)
  • Jalanko, Hannu (författare)
  • Betsholtz, Christer (författare)
  • Tryggvason, Karl (författare)
  • KTHProteomik (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of the American Society of Nephrology: Ovid Technologies (Wolters Kluwer Health)18:3, s. 689-6971046-66731533-3450

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