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Deep, high contrast...
Deep, high contrast microscopic cell imaging using three-photon luminescence of beta-(NaYF4:Er3+/NaYF4) nanoprobe excited by 1480-nm CW laser of only 1.5-mW
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Liu, Jing (författare)
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Wu, Ruitao (författare)
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Li, Nana (författare)
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Zhang, Xin (författare)
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Zhan, Qiuqiang (författare)
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- He, Sailing (författare)
- KTH,Elektroteknisk teori och konstruktion,Zhejiang University, China
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(creator_code:org_t)
- 2015
- 2015
- Engelska.
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Ingår i: Biomedical Optics Express. - 2156-7085. ; 6:5, s. 1857-1866
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- It is challenging to achieve deep microscopic imaging for the strong scattering in biotissue. An efficient three-photon luminescence can effectively increase the penetration depth. Here we report that beta-NaYF4: Er3+/NaYF4 UCNPs were excited by a 1480-nm CW-laser and emitted 543/653-nm light through a three-photon process. With the merit of the hexagonal crystal phase, sub-milliwatt laser power was utilized to excite the UCNP-probed cells to minimize the heating effect. The polymer-coated UCNPs were shown to be harmless to cells. The deep, high contrast in vitro microscopic imaging was implemented through an artificial phantom. Imaging depth of 800 mu m was achieved using only 1.5 mW excitation and a 0.7 NA objective. The green/red emission intensities ratio after penetrating the phantom was studied, indicating that longer emission wavelength is preferred for deep multiphoton microscopy. The proposed and demonstrated beta-UCNPs would have great potential in three-photon microscopy. (C) 2015 Optical Society of America
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- Up-Conversion Nanoparticles
- Upconverting Nanoparticles
- Multiphoton Microscopy
- High-Resolution
- NM
- Excitation
- Nanocrystals
- Lanthanide
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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