Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7

QC 20150617
T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.

Gustafsson, KarinKTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1ucx9jd (author)
Göthlin, MårtenKTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)- (author)
Gertow, Jens (author)
Buggert, MarcusKarolinska Institutet (author)
Frisk, Thomas W.KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1l00se6 (author)
Karlsson, Annika C.Karolinska Institutet (author)
Uhlin, MichaelKarolinska Institutet (author)
Önfelt, BjörnKTH,Cellens fysik,Science for Life Laboratory, SciLifeLab,Karolinska Institutet, Sweden(Swepub:kth)u1tquq43 (author)
Karolinska InstitutetTillämpad fysik (creator_code:org_t)

By the author/editor: Tauriainen, Joha ...; Gustafsson, Kari ...; Göthlin, Mårten; Gertow, Jens; Buggert, Marcus; Frisk, Thomas W.; show more...; Karlsson, Annika ...; Uhlin, Michael; Önfelt, Björn; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Immunology

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