NMR studies of membranes composed of glycolipids and phospholipids

• Lipid membranes composed of monogalactosyldiacylglycerol (MGDG) and dimyristoylphosphatidylcholine (DMPC) were studied by means of NMR spectroscopy. The macroscopic phase behaviour was investigated by P-31 NMR under stationary conditions, whereas microscopic properties such as segmental ordering were probed by two-dimensional H-1-C-13 separated local field experiments under magic-angle spinning conditions. Our results clearly show that ordering/disordering effects occur for the headgroups as well as for the acyl chains when the sample composition is varied. In particular, the H-1-C-13 dipolar couplings within the galactose headgroup of MGDG exhibited significant concentration dependence.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

solid-state NMR

carbon-13

phosphorus-31

dipolar couplings

separated local field

lyotropic liquid crystals

biomembranes

nuclear-magnetic-resonance

heteronuclear dipolar couplings

field-cycling nmr

biological-membranes

lipid-bilayers

model membranes

mas nmr

monogalactosyl diglyceride

cross-polarization

segmental order

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