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  • Eriksson, JensStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut (author)

Characterization of motility and piliation in pathogenic Neisseria

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • 2015-04-30
  • Springer Science and Business Media LLC,2015
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-172260
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-172260URI
  • https://doi.org/10.1186/s12866-015-0424-6DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-118549URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20150819
  • Background: The type IV pili (Tfp) of pathogenic Neisseria (i. e., N. gonorrhoeae and N. meningitidis) are essential for twitching motility. Tfp retraction, which is dependent on the ATPase PilT, generates the forces that move bacteria over surfaces. Neisseria motility has mainly been studied in N. gonorrhoeae whereas the motility of N. meningitidis has not yet been characterized. Results: In this work, we analyzed bacterial motility and monitored Tfp retraction using live- cell imaging of freely moving bacteria. We observed that N. meningitidis moved over surfaces at an approximate speed of 1.6 mu m/s, whereas N. gonorrhoeae moved with a lower speed (1.0 mu/s). An alignment of the meningococcal and gonococcal pilT promoters revealed a conserved single base pair variation in the -10 promoter element that influence PilT expression. By tracking mutants with altered pilT expression or pilE sequence, we concluded that the difference in motility speed was independent of both. Live-cell imaging using total internal reflection fluorescence microscopy demonstrated that N. gonorrhoeae more often moved with fewer visible retracting filaments when compared to N. meningitidis. Correspondingly, meningococci also displayed a higher level of piliation in transmission electron microscopy. Nevertheless, motile gonococci that had the same number of filaments as N. meningitidis still moved with a lower speed. Conclusions: These data reveal differences in both speed and piliation between the pathogenic Neisseria species during twitching motility, suggesting a difference in Tfp-dynamics.

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  • Eriksson, Olaspers SaraStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)saer5592 (author)
  • Maudsdotter, LisaStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut (author)
  • Palm, OskarKTH,Statistisk fysik(Swepub:kth)u1vx0ee5 (author)
  • Engman, JakobStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)jengm (author)
  • Sarkissian, TimStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut (author)
  • Aro, HelenaStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)haro (author)
  • Wallin, MatsKTH,Statistisk fysik(Swepub:kth)u1c9fwsv (author)
  • Jonsson, Ann-BethStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)anjons (author)
  • Stockholms universitetInstitutionen för molekylär biovetenskap, Wenner-Grens institut (creator_code:org_t)

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  • In:BMC Microbiology: Springer Science and Business Media LLC151471-2180

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