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Micro-droplet based...
Micro-droplet based directed evolution outperforms conventional laboratory evolution
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- Sjöström, Staffan (author)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Huang, Mingtao, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nielsen, Jens (author)
- KTH,Science for Life Laboratory, SciLifeLab,Proteomik och nanobioteknologi,Chalmers tekniska högskola,Chalmers University of Technology
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- Jönsson, Håkan (author)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Andersson Svahn, Helene (author)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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(creator_code:org_t)
- ISBN 9780979806476
- Chemical and Biological Microsystems Society, 2014
- 2014
- English.
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In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. - : Chemical and Biological Microsystems Society. - 9780979806476 ; , s. 169-171
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Abstract
Subject headings
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- We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Cell factories
- Directed evolution
- Droplet microfluidics
- Enzymes
- High throughput
- Cytology
- Drops
- Microfluidics
- Alpha-amylase activity
- Directed evolution methods
- Industrial enzymes
- Micro droplets
- Microfluidic droplets
- Cells
Publication and Content Type
- ref (subject category)
- kon (subject category)
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