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Host-derived pericy...
Host-derived pericytes and Sca-1+ cells predominate in the MART-1− stroma fraction of experimentally induced melanoma
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- Treviño-Villareal, J Humberto (author)
- Dept of Environmental Health, Harvard School of Public Health
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- Cotanche, Douglas A (author)
- Dept of Environmental Health, Harvard School of Public Health
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- Sepúlveda, Rosalinda (author)
- Dept of Environmental Health, Harvard School of Public Health
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- Bortoni, Magda E (author)
- Dept of Environmental Health, Harvard School of Public Health
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- Manneberg, Otto, 1981- (author)
- Dept of Environmental Health, Harvard School of Public Health
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- Udagawa, Taturo (author)
- Vertex Pharmaceuticals, Cambridge, Massachusetts
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- Rogers, Rick A (author)
- Dept of Environmental Health, Harvard School of Public Health
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(creator_code:org_t)
- 2011-12-05
- 2011
- English.
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In: Journal of Histochemistry and Cytochemistry. - : SAGE Publications. - 0022-1554 .- 1551-5044. ; 59, s. 1060-1075
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Abstract
Subject headings
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- Identification of cell types in tumor-associated stroma that are involved in the development of melanoma is hampered by their heterogeneity. The authors used flow cytometry and immunohistochemistry to demonstrate that anti-MART-1 antibodies can discriminate between melanoma and stroma cells. They investigated the cellular composition of the MART-1-, non-hematopoietic melanoma-associated stroma, finding it consisted mainly of Sca-1+ and CD146+ cells. These cell types were also observed in the skin and muscle adjacent to developing melanomas. The Sca-1+ cell population was observed distributed in the epidermis, hair follicle bulges, and tumor capsule. The CD146+ population was found distributed within the tumor, mainly associated with blood vessels in a perivascular location. In addition to a perivascular distribution, CD146+ cells expressed α-smooth muscle actin, lacked expression of endothelial markers CD31 and CD34, and were therefore identified as pericytes. Pericytes were found to be associated with CD31+ endothelial cells; however, some pericytes were also observed associated with CD31-, MART-1+ B16 melanoma cells that appeared to form blood vessel structures. Furthermore, the authors observed extensive nuclear expression of HIF-1α in melanoma and stroma cells, suggesting hypoxia is an important factor associated with the melanoma microenvironment and vascularization. The results suggest that pericytes and Sca-1+ stroma cells are important contributors to melanoma development.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- MART-1
- Sca-1
- CD146
- pericytes
- melanoma
- tumor-associated stroma
Publication and Content Type
- ref (subject category)
- art (subject category)
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