Search: onr:"swepub:oai:DiVA.org:kth-17951" >
MAP20, a Microtubul...
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Rajangam, Alex S.KTH,Strategiskt Centrum för Biomimetiska Material, BioMime
(author)
MAP20, a Microtubule-Associated Protein in the Secondary Cell Walls of Hybrid Aspen, Is a Target of the Cellulose Synthesis Inhibitor 2,6-Dichlorobenzonitrile
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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2008-09-19
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Oxford University Press (OUP),2008
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-17951
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-17951URI
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https://doi.org/10.1104/pp.108.121913DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20100525
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We have identified a gene, denoted PttMAP20, which is strongly up-regulated during secondary cell wall synthesis and tightly coregulated with the secondary wall-associated CESA genes in hybrid aspen (Populus tremula x tremuloides). Immunolocalization studies with affinity-purified antibodies specific for PttMAP20 revealed that the protein is found in all cell types in developing xylem and that it is most abundant in cells forming secondary cell walls. This PttMAP20 protein sequence contains a highly conserved TPX2 domain first identified in a microtubule-associated protein (MAP) in Xenopus laevis. Overexpression of PttMAP20 in Arabidopsis (Arabidopsis thaliana) leads to helical twisting of epidermal cells, frequently associated with MAPs. In addition, a PttMAP20-yellow fluorescent protein fusion protein expressed in tobacco (Nicotiana tabacum) leaves localizes to microtubules in leaf epidermal pavement cells. Recombinant PttMAP20 expressed in Escherichia coli also binds specifically to in vitro-assembled, taxol-stabilized bovine microtubules. Finally, the herbicide 2,6-dichlorobenzonitrile, which inhibits cellulose synthesis in plants, was found to bind specifically to PttMAP20. Together with the known function of cortical microtubules in orienting cellulose microfibrils, these observations suggest that PttMAP20 has a role in cellulose biosynthesis.
Subject headings and genre
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carbohydrate-active enzymes
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plant functional genomics
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kinesin-like
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protein
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cortical microtubules
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plasma-membrane
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wood formation
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arabidopsis-thaliana
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conifer tracheids
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angiosperm trees
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urea solution
Added entries (persons, corporate bodies, meetings, titles ...)
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Kumar, Manoj
(author)
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Aspeborg, HenrikKTH,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u19xuty6
(author)
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Guerriero, GeaKTH,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1p3u22n
(author)
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Arvestad, LarsKTH,Beräkningsbiologi, CB(Swepub:kth)u104ogsm
(author)
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Pansri, PodjamasKTH,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)PI000000
(author)
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Brown, Christian J. L.KTH,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1jzkywx
(author)
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Hober, SophiaKTH,Proteomik(Swepub:kth)u11qqzc1
(author)
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Blomqvist, KristinaKTH,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1k2zyo0
(author)
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Divne, ChristinaKTH,Glykovetenskap,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1mvd1wz
(author)
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Ezcurra, InésKTH,Glykovetenskap,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1sdw81m
(author)
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Mellerowicz, Ewa
(author)
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Sundberg, Bjorn
(author)
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Bulone, VincentKTH,Glykovetenskap,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u13o1auq
(author)
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Teeri, Tuula T.KTH,Glykovetenskap,Strategiskt Centrum för Biomimetiska Material, BioMime(Swepub:kth)u1vcejiv
(author)
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KTHStrategiskt Centrum för Biomimetiska Material, BioMime
(creator_code:org_t)
Related titles
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In:Plant Physiology: Oxford University Press (OUP)148:3, s. 1283-12940032-08891532-2548
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- By the author/editor
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Rajangam, Alex S ...
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Kumar, Manoj
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Aspeborg, Henrik
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Guerriero, Gea
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Arvestad, Lars
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Pansri, Podjamas
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show more...
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Brown, Christian ...
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Hober, Sophia
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Blomqvist, Krist ...
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Divne, Christina
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Ezcurra, Inés
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Mellerowicz, Ewa
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Sundberg, Bjorn
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Bulone, Vincent
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Teeri, Tuula T.
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- Articles in the publication
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Plant Physiology
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Royal Institute of Technology