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Stabilization of an...
Stabilization of an amine transaminase for biocatalysis
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- Chen, Shan (author)
- KTH,Industriell bioteknologi
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- Land, Henrik (author)
- KTH,Industriell bioteknologi
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- Berglund, Per (author)
- KTH,Industriell bioteknologi
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- Svedendahl Humble, Maria (author)
- KTH,Industriell bioteknologi
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(creator_code:org_t)
- Elsevier, 2016
- 2016
- English.
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In: Journal of Molecular Catalysis B. - : Elsevier. - 1381-1177 .- 1873-3158. ; 124, s. 20-28
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The amine transaminase from Chromobacterium violaceum (Cv-ATA) is a well-known enzyme to achievechiral amines of high enantiomeric excess in laboratory scales. However, the low operational stabilityof Cv-ATA limits the enzyme applicability on larger scales. In order to improve the operational stabilityof Cv-ATA, and thereby extending its applicability, factors (additives, co-solvents, organic solvents anddifferent temperatures) targeting enzyme stability and activity were explored in order to find out how tostore and apply the enzyme. The present investigation shows that the melting point of Cv-ATA is improvedby adding sucrose or glycerol, separately. Further, by storing the enzyme at higher concentrations and inco-solvents, such as; 50% glycerol, 20% methanol or 10% DMSO, the active dimeric structure of Cv-ATAis retained. Enzyme stored in 50% glycerol at −20◦C was e.g., still fully active after 6 months. Finally,the enzyme performance was improved 5-fold by a co-lyophilization with surfactants prior to usage inisooctane.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Keyword
- Bioteknologi
- Biotechnology
Publication and Content Type
- ref (subject category)
- art (subject category)
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