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Knockdown of TRPC3 ...
Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells
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- Lanner, Johanna T. (författare)
- Karolinska Institutet
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- Bruton, Joseph D. (författare)
- Karolinska Institutet
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- Assefaw-Redda, Yohannes (författare)
- KTH,Integrerade komponenter och kretsar
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Andronache, Zoita (författare)
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Severa, Denise (författare)
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- Zhang, Zhibin (författare)
- KTH,Integrerade komponenter och kretsar
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Melzer, Werner (författare)
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- Zhang, Shi-Li (författare)
- KTH,Integrerade komponenter och kretsar
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- Katz, Abram (författare)
- Karolinska Institutet
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- Westerblad, Hakan (författare)
- Karolinska Institutet
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- Zhang, SJ (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2009-01-13
- 2009
- Engelska.
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Ingår i: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 23:6, s. 1728-1738
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The involvement of Ca2+ in the insulin-mediated signaling cascade, resulting in glucose uptake in skeletal muscle, is uncertain. Here, we test the hypothesis that Ca2+ influx through canonical transient receptor potential 3 (TRPC3) channels modulates insulin-mediated glucose uptake in adult skeletal muscle. Experiments were performed on adult skeletal muscle cells of wild-type (WT) and obese, insulin-resistant ob/ob mice. Application of the diacylglycerol analog 1-oleyl-2-acetyl-sn-glycerol (OAG) induced a nonselective cation current, which was inhibited by the addition of anti-TRPC3 antibody in the patch pipette and smaller in ob/ob than in WT cells. Knockdown of TRPC3, using a novel technique based on small interfering RNA (siRNA) coupled to functionalized carbon nanotubes, resulted in pronounced (similar to 70%) decreases in OAG-induced Ca2+ influx and insulin-mediated glucose uptake. TRPC3 and the insulin-sensitive glucose transporter 4 (GLUT4) coimmunoprecipitated, and immunofluorescence staining showed that they were colocalized in the proximity of the transverse tubular system, which is the predominant site of insulin-mediated glucose transport in skeletal muscle. In conclusion, our results indicate that TRPC3 interacts functionally and physically with GLUT4, and Ca2+ influx through TRPC3 modulates insulin-mediated glucose uptake. Thus, TRPC3 is a potential target for treatment of insulin-resistant conditions.-Lanner, J. T., Bruton, J. D., Assefaw-Redda, Y., Andronache, Z., Zhang, S.- J., Severa, D., Zhang, Z.- B., Melzer, W., Zhang, S.-L., Katz, A., Westerblad, H. Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells. FASEB J. 23, 1728-1738 (2009)
Nyckelord
- Ca2
- sarcolemma
- transverse tubules
- near-membrane Ca2
- colocalization
- protein-kinase-c
- stimulated glut4 translocation
- cation channels
- transverse tubules
- plasma-membrane
- calcium sensor
- vesicle fusion
- slow-twitch
- ca2+ influx
- activation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Lanner, Johanna ...
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Bruton, Joseph D ...
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Assefaw-Redda, Y ...
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Andronache, Zoit ...
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Severa, Denise
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Zhang, Zhibin
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visa fler...
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Melzer, Werner
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Zhang, Shi-Li
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Katz, Abram
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Westerblad, Haka ...
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Zhang, SJ
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visa färre...
- Artiklar i publikationen
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The FASEB Journa ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Karolinska Institutet