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Affinity maturation...
Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules
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- von Schantz, Laura (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Dept of Immunotechnology, Lund University, Lund, Sweden
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- Gullfot, Fredrika (författare)
- KTH,Glykovetenskap,School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden
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- Scheer, Sebastian (författare)
- Dept of Immunotechnology, Lund University, Lund, Sweden; The International Max Plank Research School for Molecular and Cellular Biology, Max-Planck-Institute of Immunobiology, Freiburg, Germany
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- Filonova, Lada (författare)
- Dept of Wood Science, Swedish University of Agricultural Science, Uppsala, Sweden; WURC, Swedish University of Agricultural Science, Uppsala, Sweden
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- Cicortas Gunnarsson, Lavinia (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Dept of Immunotechnology, Lund University, Lund, Sweden; Affitech AS, Oslo, Norway
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- Flint, James E. (författare)
- Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
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- Daniel, Geoffrey (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skogens produkter,Department of Forest Products,Dept of Wood Science, Swedish University of Agricultural Science, Uppsala, Sweden; WURC, Swedish University of Agricultural Science, Uppsala, Sweden
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- Nordberg Karlsson, Eva (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Dept of Biotechnology, Lund University, Lund, Sweden
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- Brumer, Harry (författare)
- KTH,Glykovetenskap,School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden
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- Ohlin, Mats (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Dept of Immunotechnology, Lund University, Lund, Sweden
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- Stålhandske, Lada (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Trävetenskap,Forest economy
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(creator_code:org_t)
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- 2009-10-31
- 2009
- Engelska.
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Ingår i: BMC Biotechnology. - : Springer Science and Business Media LLC. - 1472-6750. ; 9
- Relaterad länk:
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https://bmcbiotechno...
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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https://lup.lub.lu.s...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Molecular evolution of carbohydrate binding modules (CBM) is a new approach for the generation of glycan-specific molecular probes. To date, the possibility of performing affinity maturation on CBM has not been investigated. In this study we show that binding characteristics such as affinity can be improved for CBM generated from the CBM4-2 scaffold by using random mutagenesis in combination with phage display technology. Results: Two modified proteins with greatly improved affinity for xyloglucan, a key polysaccharide abundant in the plant kingdom crucial for providing plant support, were generated. Both improved modules differ from other existing xyloglucan probes by binding to galactose-decorated subunits of xyloglucan. The usefulness of the evolved binders was verified by staining of plant sections, where they performed better than the xyloglucan-binding module from which they had been derived. They discriminated non-fucosylated from fucosylated xyloglucan as shown by their ability to stain only the endosperm, rich in non-fucosylated xyloglucan, but not the integument rich in fucosylated xyloglucan, on tamarind seed sections. Conclusion: We conclude that affinity maturation of CBM selected from molecular libraries based on the CBM4-2 scaffold is possible and has the potential to generate new analytical tools for detection of plant carbohydrates.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Skogsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Forest Science (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- plant-cell walls
- somatic hypermutation
- protein stability
- antibodies
- selection
- recognition
- diversity
- mechanism
- evolution
- display
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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