Sökning: L773:0041 0101 OR L773:1879 3150 >
Kinetic evidence fo...
-
Jolkkonen, Mikael
(författare)
Kinetic evidence for different mechanisms of interaction of black mamba toxins MT alpha and MT beta with muscarinic receptors
- Artikel/kapitelEngelska2001
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:kth-20049
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-20049URI
-
https://doi.org/10.1016/S0041-0101(00)00141-0DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
QC 20100525
-
By studying the influence of two toxins from the black mamba Dendroaspis polylepis on the kinetics of [H-3]-N-methylscopolamine binding to muscarinic acetylcholine receptors from rat cerebral cortex, it was revealed that these toxins, MT alpha and MT beta, interact with the receptors via kinetically distinct mechanisms. MT beta bound to receptors in a one-step, readily reversible process with the dissociation constant K-d = 5.3 mu M. The binding mechanism of MT alpha was more complex, involving at least two consecutive steps. A fast receptor-toxin complex formation (K-T = 3.8 mu M) was followed by a slow process of isomerisation of this complex (k(i) = 1.8 x 10(-2) s(-1), half-time 39 s). A similar two-step interaction mechanism has been established for a related toxin, MT2 from the green mamba D. angusticeps (K-T = 1.4 mu M, k(i) = 8.3 x 10(-4) s(-1), half-time 840 s). The slow isomerisation process delays the effect of MT alpha and MT2, but increases their apparent potency compared to toxins unable to induce the isomerisation process.
Ämnesord och genrebeteckningar
-
snake toxins (Dendroaspis polylepis)
-
muscarinic acetylcholine
-
receptor
-
muscarinic toxin
-
binding mechanism
-
amino-acid-sequence
-
acetylcholine-receptors
-
binding
-
antagonist
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Oras, A.
(författare)
-
Toomela, T.
(författare)
-
Karlsson, E.
(författare)
-
Jarv, J.
(författare)
-
Akerman, K. E. O.
(författare)
Sammanhörande titlar
-
Ingår i:Toxicon39:2-3, s. 377-3820041-01011879-3150
Internetlänk
Hitta via bibliotek
-
Toxicon
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas