Search: id:"swepub:oai:DiVA.org:kth-202878" >
Analysis of Body-wi...
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Butler, L. M.Karolinska Institutet
(author)
Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome
- Article/chapterEnglish2016
Publisher, publication year, extent ...
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Cell Press,2016
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-202878
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-202878URI
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https://doi.org/10.1016/j.cels.2016.08.001DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-318136URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:135241232URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20170310
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Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hallström, Björn MikaelKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Royal Inst Technol KTH, Sch Biotechnol, Sci Life Lab, S-17121 Stockholm, Sweden(Swepub:kth)u1ih19oh
(author)
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Fagerberg, LinnKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Royal Inst Technol KTH, Sch Biotechnol, Sci Life Lab, S-17121 Stockholm, Sweden(Swepub:kth)u1dglfid
(author)
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Pontén, Fredrik CUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)fredpont
(author)
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Uhlén, MathiasKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Royal Inst Technol KTH, Sch Biotechnol, Sci Life Lab, S-17121 Stockholm, Sweden(Swepub:kth)u1dulvmw
(author)
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Renné, T.Karolinska Institutet
(author)
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Odeberg, JacobKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Royal Inst Technol KTH, Sch Biotechnol, Sci Life Lab, S-17121 Stockholm, Sweden.; Karolinska Univ Hosp, Coagulat Unit, Ctr Hematol, S-17176 Stockholm, Sweden(Swepub:kth)u1e4yljo
(author)
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Karolinska InstitutetProteomik och nanobioteknologi
(creator_code:org_t)
Related titles
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In:Cell Systems: Cell Press3:3, s. 287-301.e32405-4712
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