Search: id:"swepub:oai:DiVA.org:kth-205137" >
Specific Arabidopsi...
-
Beatriz Badia, Mariana
(author)
Specific Arabidopsis thaliana malic enzyme isoforms can provide anaplerotic pyruvate carboxylation function in Saccharomyces cerevisiae
- Article/chapterEnglish2017
Publisher, publication year, extent ...
-
2017-02-01
-
WILEY,2017
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:kth-205137
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-205137URI
-
https://doi.org/10.1111/febs.14013DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
QC 20170412
-
NAD(P)-malic enzyme (NAD(P)-ME) catalyzes the reversible oxidative decarboxylation of malate to pyruvate, CO2, and NAD(P)H and is present as a multigene family in Arabidopsis thaliana. The carboxylation reaction catalyzed by purified recombinant Arabidopsis NADP-ME proteins is faster than those reported for other animal or plant isoforms. In contrast, no carboxylation activity could be detected in vitro for the NAD-dependent counterparts. In order to further investigate their putative carboxylating role in vivo, Arabidopsis NAD(P)-ME isoforms, as well as the NADP-ME2del2 (with a decreased ability to carboxylate pyruvate) and NADP-ME2R115A (lacking fumarate activation) versions, were functionally expressed in the cytosol of pyruvate carboxylase-negative (Pyc(-)) Saccharomyces cerevisiae strains. The heterologous expression of NADP-ME1, NADP-ME2 (and its mutant proteins), and NADP-ME3 restored the growth of Pyc(-) S. cerevisiae on glucose, and this capacity was dependent on the availability of CO2. On the other hand, NADP-ME4, NAD-ME1, and NAD-ME2 could not rescue the Pyc(-) strains from C-4 auxotrophy. NADP-ME carboxylation activity could be measured in leaf crude extracts of knockout and over-expressing Arabidopsis lines with modified levels of NADP-ME, where this activity was correlated with the amount of NADP-ME2 transcript. These results indicate that specific A. thaliana NADP-ME isoforms are able to play an anaplerotic role in vivo and provide a basis for the study on the carboxylating activity of NADP-ME, which may contribute to the synthesis of C-4 compounds and redox shuttling in plant cells.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Mans, Robert
(author)
-
Lis, Alicia V.
(author)
-
Ariel Tronconi, Marcos
(author)
-
Lucia Arias, Cintia
(author)
-
Maurino, Veronica Graciela
(author)
-
Santiago Andreo, Carlos
(author)
-
Fabiana Drincovich, Maria
(author)
-
van Maris, Antonius J. A.KTH,Industriell bioteknologi(Swepub:kth)u1n330r1
(author)
-
Gerrard Wheeler, Mariel Claudia
(author)
-
KTHIndustriell bioteknologi
(creator_code:org_t)
Related titles
-
In:The FEBS Journal: WILEY284:4, s. 654-6651742-464X1742-4658
Internet link
Find in a library
To the university's database