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Triple helix of bet...
Triple helix of beta-D-glucan from Lentinus Edodes in 0.5 M NaCl aqueous solution characterized by light scattering
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Zhang, L. N. (författare)
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Zhang, X. F. (författare)
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- Zhou, Qi (författare)
- KTH,Bioteknologi
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Zhang, P. Y. (författare)
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Zhang, M. (författare)
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Li, X. L. (författare)
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2001
- 2001
- Engelska.
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Ingår i: Polymer journal. - : Springer Science and Business Media LLC. - 0032-3896 .- 1349-0540. ; 33:4, s. 317-321
- Relaterad länk:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- beta-(1 -->3)-D-glucan with (1 -->6) branching (L-FV-I) from Lentinus edodes in water was degraded into seven fractions of different molecular weights by ultrasonic irradiation. Weight-average molecular weight M,, radius of gyration (1/2)(5) and intrinsic viscosity [eta] of the beta -D-glucan and its fractions in 0.5 M NaCl aqueous solution and dimethylsulfoxide (DMSO) were studied by multi-angle laser light scattering (MALLS), GPC combined with MALLS, and viscometry. M, dependence of [eta] for the glucan in 0.5 M NaCl aqueous solution was represented approximately by [eta] =7.69 x 10(-6) M-w(1.32) (cm(3) g(-1)) at M-w from 1.87 X 10(5) to 1.20 X 10(6) at 25 degreesC. GPC chromatograms of the glucans in aqueous solution contained two peaks, a main peak corresponding to triple-stranded chains with molecular weight M-w,M-m, and small second peak corresponding to fragments of single chains with M-w,M-s (about 20 +/-5% content). Analysis of M-w,M-m and (1/2)(z,m) in term of the known theory for wormlike chains yielded 2180 +/- 100 nm(-1), 120 +/- 10 nm and 0.31 nm for molar mass per unit contour length M-L, persistence length q, and contour length h per main-chain glucose residue, respectively, which agree closely with theory data of triple-helical chains and reported parameters for triple-helix schizophyllan in 0.01 M NaOH aqueous solution. The ratios of M-w,M-m, in 0.5 M NaCl to M, in DMSO were calculated to be roughly 3. The predominant species of the glucan in 0.5 M NaCl aqueous solution exist as triple-helical chains with high rigidity, and in DMSO as single-flexible chains.
Nyckelord
- lentinan
- beta-D-glucan
- molecular weight
- intrinsic viscosity
- conformation
- triple-helix chain
- light scattering
- gel permeation chromatopgraphy
- schizophyllum-commune polysaccharide
- cell-mediated responses
- state c-13 nmr
- antitumor-activity
- sodium-hydroxide
- dilute-solution
- branched (1->3)-beta-d-glucans
- conformation
- viscosity
- rigidity
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